ArticleJournal of clinical microbiology2018
Interlaboratory Comparison of Six Real-Time PCR Assays for Detection of Bovine Leukemia Virus Proviral DNA.
Article in Journal of clinical microbiology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed.
- Droplet digital PCR (ddPCR) for enhanced detection and quantification of bovine viral diarrhea virus (BVDV): A promising tool for disease control.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2025Article
- Detection of Bovine Leukemia Virus in Argentine, Bolivian, Paraguayan and Cuban Native Cattle Using a Quantitative Real-Time PCR Assay-BLV-CoCoMo-qPCR-2.Pathogens (Basel, Switzerland) · 2025Article
- Experimental reporting of fish transcriptomic responses in environmental toxicology and ecotoxicology.Environmental toxicology and chemistry · 2025Review
- Polymorphism ofPathogens (Basel, Switzerland) · 2025Article
- When Two-Fold Is Not Enough: Quantifying Uncertainty in Low-Copy qPCR.International journal of molecular sciences · 2025Article
- Development and application of a quantitative polymerase chain reaction assay for the detection and genotyping of bovine leukemia virus in cattle from Kazakhstan.Veterinary world · 2025Article
- Article
- BLV-CoCoMo Dual qPCR Assay Targeting LTR Region for Quantifying Bovine Leukemia Virus: Comparison with Multiplex Real-Time qPCR Assay TargetingPathogens (Basel, Switzerland) · 2024Article
- Reduction of antisense transcription affects bovine leukemia virus replication and oncogenesis.PLoS pathogens · 2024Article
- Inter-laboratory comparison of eleven quantitative or digital PCR assays for detection of proviral bovine leukemia virus in blood samples.BMC veterinary research · 2024Article
- Article
- The Global Epidemiology of Bovine Leukemia Virus: Current Trends and Future Implications.Animals : an open access journal from MDPI · 2024Review
- Bovine leukemia virus encoded blv-miR-b4-3p microRNA is associated with reduced expression of anti-oncogenic gene in vivo.PloS one · 2023Article
- Comparative Evaluation of Three Commercial Quantitative Real-Time PCRs Used in Japan for Bovine Leukemia Virus.Viruses · 2022Article
- Development of a droplet digital PCR assay for quantification of the proviral load of bovine leukemia virus.Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc · 2022Article
- A safe and effective vaccine against bovine leukemia virus.Frontiers in immunology · 2022Article
- Tracing Viral Transmission and Evolution of Bovine Leukemia Virus through Long Read Oxford Nanopore Sequencing of the Proviral Genome.Pathogens (Basel, Switzerland) · 2021Article
- TB or not TB? Definitive determination of species within the Mycobacterium tuberculosis complex in unprocessed sputum from adults with presumed multidrug-resistant tuberculosis.Tropical medicine & international health : TM & IH · 2021Article
- Molecular Characterization of thePathogens (Basel, Switzerland) · 2021Article
- Quantitative PCR of Small Nucleic Acids: Size Matters.ChemistrySelect · 2021Article
Corrections and comments
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Authors and funding
14 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Quantitative real-time PCR (qPCR) is increasingly being used for the detection of bovine leukemia virus (BLV) proviral DNA. Nevertheless, quality control for the validation and standardization of such tests is currently lacking. Therefore, the present study was initiated by three Office International des Epizooties (OIE) reference laboratories and three collaborating laboratories to measure the interlaboratory variability of six already developed and available BLV qPCR assays. For that purpose, an international panel of 58 DNA samples reflecting the dynamic range of the majority of the assays was distributed to six testing centers. Based on qualitative results, the overall agreement among all six laboratories was moderate. However, significant variability in the measurement of the BLV proviral DNA copy number was observed among different laboratories. Quantitative PCR assays, even when performed by experienced staff, can yield large variability in BLV proviral DNA copy numbers without harmonization. Further standardization of different factors (i.e., utilization of unified protocols and unique calibrators) should increase interlaboratory agreement.
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