Evidence map›Paper›PMID 29669525›Full record

ArticleBMC cancer2018

Salivary extracellular vesicle-associated miRNAs as potential biomarkers in oral squamous cell carcinoma.

Chiara Gai, Francesco Camussi, Roberto Broccoletti, Alessio Gambino, Marco Cabras, Luca Molinaro, Stefano Carossa, Giovanni Camussi, Paolo G Arduino

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC cancer, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 120 papers, 6 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
120citing papers in PubMed, 6 pooled it
7.1field-weighted citation impact, top 2% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

120 citing papers in PubMed, 6 syntheses or guidelines pooled it, 188 citations in OpenAlex.

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60 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 1 institution in 1 country.

Chiara GaiDepartment of Medical Sciences, University of Turin, C.so Dogliotti, 14 -10126, Turin, Italy.
Francesco CamussiDepartment of Surgical Sciences, University of Turin, Via Nizza 230, 10126, Turin, Italy.
Roberto BroccolettiDepartment of Surgical Sciences, University of Turin, Via Nizza 230, 10126, Turin, Italy.
Alessio GambinoDepartment of Surgical Sciences, University of Turin, Via Nizza 230, 10126, Turin, Italy.
Marco CabrasDepartment of Surgical Sciences, University of Turin, Via Nizza 230, 10126, Turin, Italy.
Luca MolinaroDepartment of Medical Sciences, University of Turin, C.so Dogliotti, 14 -10126, Turin, Italy.
Stefano CarossaDepartment of Surgical Sciences, University of Turin, Via Nizza 230, 10126, Turin, Italy.
Giovanni CamussiDepartment of Medical Sciences, University of Turin, C.so Dogliotti, 14 -10126, Turin, Italy.
Paolo G ArduinoDepartment of Surgical Sciences, University of Turin, Via Nizza 230, 10126, Turin, Italy. paologiacomo.arduino@unito.it.
University of Turin · IT

Funding

AIRC Italy AIRC IG 2015.16973
6 · The paper itself

Abstract

backgroundSeveral studies in the past have investigated the expression of micro RNAs (miRNAs) in saliva as potential biomarkers. Since miRNAs associated with extracellular vesicles (EVs) are known to be protected from enzymatic degradation, we evaluated whether salivary EVs from patients with oral squamous cell carcinoma (OSCC) were enriched with specific subsets of miRNAs.

methodsOSCC patients and controls were matched with regards to age, gender and risk factors. Total RNA was extracted from salivary EVs and the differential expression of miRNAs was evaluated by qRT-PCR array and qRT-PCR. The discrimination power of up-regulated miRNAs as biomarkers in OSCC patients versus controls was evaluated by the Receiver Operating Characteristic (ROC) curves.

resultsA preliminary qRT-PCR array was performed on samples from 5 OSCC patients and 5 healthy controls whereby a subset of miRNAs were identified that were differentially expressed. On the basis of these results, a cohort of additional 16 patients and 6 controls were analyzed to further confirm the miRNAs that were up-regulated or selectively expressed in the previous pilot study. The following miRNAs: miR-302b-3p and miR-517b-3p were expressed only in EVs from OSCC patients and miR-512-3p and miR-412-3p were up-regulated in salivary EVs from OSCC patients compared to controls with the ROC curve showing a good discrimination power for OSCC diagnosis. The Kyoto Encyclopedia of Gene and Genomes (KEGG) pathway analysis suggested the possible involvement of the miRNAs identified in pathways activated in OSCC.

conclusionsIn this work, we suggest that salivary EVs isolated by a simple charge-based precipitation technique can be exploited as a non-invasive source of miRNAs for OSCC diagnosis. Moreover, we have identified a subset of miRNAs selectively enriched in EVs of OSCC patients that could be potential biomarkers.

Indexed as

AdultAgedBiomarkersCarcinoma, Squamous CellExtracellular VesiclesFemaleHuman papillomavirus 16HumansMaleMicroRNAsMiddle AgedMouth NeoplasmsNeoplasm StagingPapillomavirus InfectionsSalivaBiomarkersMicroRNAsExtracellular vesicles (EVs)microRNA (miRNA)miR-27a-3p (miR-27a)miR-302b-3p (miR-302b)miR-494miR-517b-3p (miR-517b)miRNA-412-3p (miR-412)miRNA-512-3p (miR-512)Oral squamous cell carcinoma (OSCC)

Identifiers

PMID29669525
PMCPMC5907383
OpenAlexW2802972251

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.