Evidence map›Paper›PMID 29666238›Full record

ArticleProceedings of the National Academy of Sciences of the United States of America2018

Rapid acquisition and model-based analysis of cell-free transcription-translation reactions from nonmodel bacteria.

Simon J Moore, James T MacDonald, Sarah Wienecke, Alka Ishwarbhai, Argyro Tsipa, Rochelle Aw, Nicolas Kylilis, David J Bell, David W McClymont, Kirsten Jensen and 3 more

Open access · bronzeAbstract read
In one paragraph

Article in Proceedings of the National Academy of Sciences of the United States of America, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 91 papers.

0numbers the graph read from it
0cells of the map it votes in
91citing papers in PubMed
11.3field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

91 citing papers in PubMed, 209 citations in OpenAlex.

  1. Review
  2. Review
  3. Article
  4. Article
  5. A Pichia pastoris Cell-Free Protein Synthesis Platform.Methods in molecular biology (Clifton, N.J.) · 2026
    Article
  6. Review
  7. Article
  8. Reconstituting alternative life using the test-bed of cell-free systems.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2025
    Review
  9. Gene circuit-based sensors.Fundamental research · 2025
    Review
  10. Article
  11. Article
  12. Review
  13. Review
  14. Review
  15. Article
  16. Review
  17. Article
  18. Article
  19. Article
  20. Review

31 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors at 3 institutions in 2 countries.

Simon J MooreCentre for Synthetic Biology and Innovation, Imperial College London, SW7 2AZ London, United Kingdom.ORCID 0000-0002-1968-206X
James T MacDonaldCentre for Synthetic Biology and Innovation, Imperial College London, SW7 2AZ London, United Kingdom.ORCID 0000-0001-9675-4429
Sarah WieneckeBraunschweig Integrated Centre of Systems Biology, Institute of Microbiology, Technische Universität Braunschweig, 38106 Braunschweig, Germany.
Alka IshwarbhaiLondon DNA Foundry, Imperial College London, SW7 2AZ London, United Kingdom.
Argyro TsipaLondon DNA Foundry, Imperial College London, SW7 2AZ London, United Kingdom.
Rochelle AwCentre for Synthetic Biology and Innovation, Imperial College London, SW7 2AZ London, United Kingdom.
Nicolas KylilisCentre for Synthetic Biology and Innovation, Imperial College London, SW7 2AZ London, United Kingdom.
David J BellSection for Structural Biology, Department of Medicine, Imperial College London, SW7 2AZ London, United Kingdom.
David W McClymontSection for Structural Biology, Department of Medicine, Imperial College London, SW7 2AZ London, United Kingdom.
Kirsten JensenCentre for Synthetic Biology and Innovation, Imperial College London, SW7 2AZ London, United Kingdom.
Karen M PolizziCentre for Synthetic Biology and Innovation, Imperial College London, SW7 2AZ London, United Kingdom.ORCID 0000-0001-5435-2667
Rebekka BiedendieckBraunschweig Integrated Centre of Systems Biology, Institute of Microbiology, Technische Universität Braunschweig, 38106 Braunschweig, Germany.
Paul S FreemontCentre for Synthetic Biology and Innovation, Imperial College London, SW7 2AZ London, United Kingdom; p.freemont@imperial.ac.uk.ORCID 0000-0002-5658-8486
London Biofoundry · GBImperial College London · GBTechnische Universität Braunschweig · DE

Funding

Biotechnology and Biological Sciences Research Council BB/F017324/1Biotechnology and Biological Sciences Research Council BB/M025632/1
6 · The paper itself

Abstract

Native cell-free transcription-translation systems offer a rapid route to characterize the regulatory elements (promoters, transcription factors) for gene expression from nonmodel microbial hosts, which can be difficult to assess through traditional in vivo approaches. One such host,

Indexed as

Bacillus megateriumModels, BiologicalProtein BiosynthesisTranscription, GeneticCell-Free SystemautomationBacilluscell-free synthetic biologyin vitro transcription–translationmodeling

Identifiers

PMID29666238
PMCPMC5948957
OpenAlexW2800282183

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.