ArticleGut2019
Genetic editing of colonic organoids provides a molecularly distinct and orthotopic preclinical model of serrated carcinogenesis.
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Article in Gut, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 76 papers.
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76citing papers in PubMed
7.8field-weighted citation impact, top 2% of its field
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Who cites it
76 citing papers in PubMed, 137 citations in OpenAlex.
- Engineering tumor-colonizing E. coli Nissle 1917 for detection and treatment of colorectal neoplasia.Nature communications · 2024Trial
- Selective Macrocyclic WEE1 Kinase Inhibitors with Strong Efficacy against Patient-Derived Colorectal Cancer Organoids.Journal of medicinal chemistry · 2026Article
- Use of in vitro co-culture models to inform bacterial engineering for the treatment of solid tumours.npj biomedical innovations · 2026Review
- Applications and limitations of pluripotent stem cell-derived liver organoids.Chinese medical journal · 2026Review
- Novel Syngeneic Cell Lines for Studying High-Risk BRAFV600E-Driven Colorectal Cancer In Vivo.Cancer research communications · 2026Article
- Patient-derived organoids (PDOs) as a promising platform for personalized treatment of colorectal cancer: current applications and future challenges.Cancer biology & therapy · 2025Review
- Single-cell sequencing and organoids: applications in organ development and disease.Molecular biomedicine · 2025Review
- Beyond Rodents: Alternative Animal Models in Colorectal Cancer Research.International journal of molecular sciences · 2025Review
- BRAFiScience · 2025Article
- Mechanistic insights into Wnt-β-catenin pathway activation and signal transduction.Nature reviews. Molecular cell biology · 2025Review
- The Nagoya University and the University of Adelaide Joint PhD Degree Program in Medicine - A Program of Opportunity.Advances in medical education and practice · 2025Article
- Evaluation of EGFR and COX pathway inhibition in human colon organoids of serrated polyposis and other hereditary cancer syndromes.Familial cancer · 2024Article
- Review
- Review
- Simple aneuploidy evades p53 surveillance and promotes niche factor-independent growth in human intestinal organoids.Molecular biology of the cell · 2024Article
- FAK loss reduces BRAFeLife · 2024Article
- Next-Generation Modeling of Cancer Using Organoids.Cold Spring Harbor perspectives in medicine · 2024Review
- TGFβ-Responsive Stromal Activation Occurs Early in Serrated Colorectal Carcinogenesis.International journal of molecular sciences · 2024Article
- A Free Amino Acid Diet Alleviates Colorectal Tumorigenesis through Modulating Gut Microbiota and Metabolites.Nutrients · 2024Article
- Lansoprazole inhibits the development of sessile serrated lesions by inducing G1 arrest via Skp2/p27 signaling pathway.Journal of gastroenterology · 2024Article
16 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
30 authors at 15 institutions in 6 countries.
Tamsin R M LannaganSchool of Medicine, University of Adelaide and South Australian Health and Medical Research Institute, Adelaide, South Australia, Australia.
Young K LeeSchool of Medicine, University of Adelaide and South Australian Health and Medical Research Institute, Adelaide, South Australia, Australia.
Tongtong WangSchool of Medicine, University of Adelaide and South Australian Health and Medical Research Institute, Adelaide, South Australia, Australia.
Jatin RoperThe David H. Koch Institute for Integrative Cancer Research at MIT, Cambridge, Massachusetts, USA.
Mark L BettingtonEnvoi Specialist Pathologists, Brisbane, Queensland, Australia.
Lochlan FennellConjoint Gastroenterology Laboratory, QIMR Berghofer Medical Research Institute, Brisbane, Queensland, Australia.
Laura VrbanacSchool of Medicine, University of Adelaide and South Australian Health and Medical Research Institute, Adelaide, South Australia, Australia.
Lisa JonaviciusDepartment of Anatomical Pathology, Flinders Medical Centre, Bedford Park, South Australia, Australia.
Roshini SomashekarSchool of Medicine, University of Adelaide and South Australian Health and Medical Research Institute, Adelaide, South Australia, Australia.
Krystyna GieniecSchool of Medicine, University of Adelaide and South Australian Health and Medical Research Institute, Adelaide, South Australia, Australia.
Miao YangSchool of Medicine, University of Adelaide and South Australian Health and Medical Research Institute, Adelaide, South Australia, Australia.
Jia Q NgSchool of Medicine, University of Adelaide and South Australian Health and Medical Research Institute, Adelaide, South Australia, Australia.
Nobumi SuzukiSchool of Medicine, University of Adelaide and South Australian Health and Medical Research Institute, Adelaide, South Australia, Australia.
Mari IchinoseSchool of Medicine, University of Adelaide and South Australian Health and Medical Research Institute, Adelaide, South Australia, Australia.
Josephine A WrightSchool of Medicine, University of Adelaide and South Australian Health and Medical Research Institute, Adelaide, South Australia, Australia.
Hiroki KobayashiSchool of Medicine, University of Adelaide and South Australian Health and Medical Research Institute, Adelaide, South Australia, Australia.
Tracey L PutoczkiDepartment of Medical Biology, University of Melbourne and the Walter and Eliza Hall Institute of Medical Research, Melbourne, Victoria, Australia.
Yoku HayakawaDepartment of Gastroenterology, University of Tokyo, Tokyo, Japan.ORCID 0000-0002-3988-2499
Simon J LeedhamTranslational Gastroenterology Unit, Experimental Medicine Division, Nuffield Department of Clinical Medicine, John Radcliffe Hospital, Oxford, UK.
Helen E AbudCancer Program, Monash Biomedicine Discovery Institute and the Department of Anatomy and Developmental Biology, Monash University, Clayton, Victoria, Australia.
Ömer H YilmazThe David H. Koch Institute for Integrative Cancer Research at MIT, Cambridge, Massachusetts, USA.
Julie MarkerCancer Voices SA, Adelaide, South Australia, Australia.
Sonja KlebeDepartment of Anatomical Pathology, Flinders Medical Centre, Bedford Park, South Australia, Australia.
Pratyaksha WirapatiBioinformatics Core Facility, Swiss Institute of Bioinformatics, Lausanne, Switzerland.
Siddhartha MukherjeeDepartment of Medicine, Columbia University Medical Center, New York City, New York, USA.
Sabine TejparDigestive Oncology Unit, Department of Oncology, University Hospitals Leuven, Leuven, Belgium.
Barbara A LeggettConjoint Gastroenterology Laboratory, QIMR Berghofer Medical Research Institute, Brisbane, Queensland, Australia.
Vicki L J WhitehallConjoint Gastroenterology Laboratory, QIMR Berghofer Medical Research Institute, Brisbane, Queensland, Australia.
Daniel L Worthley *School of Medicine, University of Adelaide and South Australian Health and Medical Research Institute, Adelaide, South Australia, Australia.
Susan L Woods *School of Medicine, University of Adelaide and South Australian Health and Medical Research Institute, Adelaide, South Australia, Australia.ORCID 0000-0002-8955-2017
South Australian Health and Medical Research Institute · AUFlinders Medical Centre · AUCentre for Human Genetics · GBColumbia University Irving Medical Center · USDiscovery Institute · USMassachusetts General Hospital · USMolecular Oncology (United States) · USQIMR Berghofer Medical Research Institute · AUQueensland Health · AUSIB Swiss Institute of Bioinformatics · CHThe University of Melbourne · AUThe University of Queensland · AUThe University of Tokyo · JPTufts Medical Center · USUniversity of the Sunshine Coast · AU
Funding
Women's Cancer Research ProgramP30CA014236 · NCI · DUKE UNIVERSITY · PI JOHN N. NORTON · 1985 to 2026
$174.8MVIRUS PRODUCTION COREP30CA014051 · NCI · MASSACHUSETTS INSTITUTE OF TECHNOLOGY · PI Jacqueline A. Lees · 1985 to 2026
$93.9MPilot & Feasibility ProgramP30DK043351 · NIDDK · MASSACHUSETTS GENERAL HOSPITAL · PI RAYMOND T CHUNG · 1991 to 2026
$35.3MUnderstanding and Improving Platinum Anticancer DrugsR01CA034992 · NCI · MASSACHUSETTS INSTITUTE OF TECHNOLOGY · PI YILMAZ, OMER · 1985 to 2018
$6.8MDietary control of stem cells in physiology and cancerR01CA211184 · NCI · MASSACHUSETTS INSTITUTE OF TECHNOLOGY · PI YILMAZ, OMER · 2017 to 2021
$2.0MThe role of Mtss1 in Hedgehog-mediated intestinal cancer stem cell functionK08CA198002 · NCI · TUFTS MEDICAL CENTER · PI ROPER, JATIN · 2015 to 2019
$845kRegulation of the Intestinal Stem Cell Niche in AgingR00AG045144 · NIA · MASSACHUSETTS INSTITUTE OF TECHNOLOGY · PI YILMAZ, OMER · 2014 to 2016
$740kReal-time in Vivo Dosimetry in Radiation Therapy Using Scintillation DetectorsR01CA120198 · NCI · UNIVERSITY OF TX MD ANDERSON CAN CTR · PI BEDDAR, SAM · 2007 to 2009
$578kCancer Research UK 16581NCI NIH HHS K08 CA198002NCI NIH HHS P30 CA014051NCI NIH HHS P30 CA014236NCI NIH HHS R01 CA034992NCI NIH HHS R01 CA120198NCI NIH HHS R01 CA211184NIA NIH HHS R00 AG045144NIDDK NIH HHS P30 DK043351Wellcome Trust 206314/Z/17/Z
Abstract
objectiveSerrated colorectal cancer (CRC) accounts for approximately 25% of cases and includes tumours that are among the most treatment resistant and with worst outcomes. This CRC subtype is associated with activating mutations in the mitogen-activated kinase pathway gene,
designWe use organoid culture combined with CRISPR/Cas9 genome engineering to sequentially introduce genetic alterations associated with serrated CRC and which regulate the stem cell niche, senescence and DNA mismatch repair.
resultsTargeted biallelic gene alterations were verified by DNA sequencing. Organoid growth in the absence of niche factors was assessed, as well as analysis of downstream molecular pathway activity. Orthotopic engraftment of complex organoid lines, but not
conclusionWe generate rapid, complex models of serrated CRC to determine the contribution of specific genetic alterations to carcinogenesis. Analysis of our models alongside patient data has led to the identification of a potential susceptibility for this tumour type.
Indexed as
AdenocarcinomaAllelesColonColorectal NeoplasmsCpG IslandsDisease ProgressionDNA Mismatch RepairDNA Mutational AnalysisEpigenomicsGene Expression Regulation, NeoplasticGenes, Tumor SuppressorHumansModels, GeneticMutationOrganoidsPhenotypeBRAF protein, humanProto-Oncogene Proteins B-rafcancer geneticscolorectal cancergene mutationmethylationoncogenes
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.