ArticleCryobiology2018
Cryopreservation of sperm bundles (spermatozeugmata) from endangered livebearing goodeids.
Article in Cryobiology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
6 citing papers in PubMed.
- Developing Efficient Methods of Sperm Cryopreservation for Three Fish Species (International journal of molecular sciences · 2025Article
- An Open-Hardware Insemination Device for Small-Bodied Live-Bearing Fishes to Support Development and Use of Germplasm Repositories.Animals : an open access journal from MDPI · 2022Article
- Development of an open hardware 3-D printed conveyor device for continuous cryopreservation of non-batched samples.Aquacultural engineering · 2021Article
- Development of germplasm repositories to assist conservation of endangered fishes: Examples from small-bodied livebearing fishes.Theriogenology · 2019Article
- The role of alkalinization-induced Ca2+ influx in sperm motility activation of a viviparous fish Redtail Splitfin (Xenotoca eiseni).Biology of reproduction · 2018Article
- Production of live young with cryopreserved sperm from the endangered livebearing fish Redtail Splitfin (Xenotoca eiseni, Rutter, 1896).Animal reproduction science · 2018Article
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
More than half of fishes in the family Goodeidae are considered to be endangered, threatened, or vulnerable. Sperm cryopreservation is an effective tool for conserving genetic resources of imperiled populations, but development of protocols with livebearing fishes faces numerous challenges including the natural packaging of sperm into bundles. In this study the cryopreservation of sperm bundles (spermatozeugmata) of three goodeids species was evaluated. Sperm quality was evaluated by activation with NaCl-NaOH solution (at 300 mOsmol/kg and pH 11.8), and analysis of dissociable bundles and dissociation duration. Using Redtail Splitfin (Xenotoca eiseni) as a model, the effects of cryoprotectants (dimethyl sulfoxide, methanol, and glycerol) with different concentrations (5-15% v/v %), equilibration exposure times (1-60 min), cooling rates (5-40 °C/min), concentrations (4 × 10
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Registered trials
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