ArticlePloS one2018
Actin and myosin II modulate differentiation of pluripotent stem cells.
Article in PloS one, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
14 citing papers in PubMed.
- Advances in engineered models of peri-gastrulation.iScience · 2025Review
- ZO-1 boosts the in vitro self-renewal of pre-haematopoietic stem cells from OCT4-reprogrammed human hair follicle mesenchymal stem cells through cytoskeleton remodeling.Stem cell research & therapy · 2024Article
- Nuclear actin is a critical regulator ofbioRxiv : the preprint server for biology · 2024Article
- Nanofiber-microwell cell culture system for spatially patterned differentiation of pluripotent stem cells in 3D.Materials today. Bio · 2024Article
- Cell Behavioral Dynamics as a Cue in Optimizing Culture Stabilization in the Bioprocessing of Pluripotent Stem Cells.Bioengineering (Basel, Switzerland) · 2022Review
- A novel alveolar epithelial cell sheet fabricated under feeder-free conditions for potential use in pulmonary regenerative therapy.Regenerative therapy · 2022Article
- Nucleus-cytoskeleton communication impacts on OCT4-chromatin interactions in embryonic stem cells.BMC biology · 2022Article
- Fluorescence-based actin turnover dynamics of stem cells as a profiling method for stem cell functional evolution, heterogeneity and phenotypic lineage parsing.Methods (San Diego, Calif.) · 2021Article
- Heading towards a dead end: The role of DND1 in germ line differentiation of human iPSCs.PloS one · 2021Article
- Pluripotency of embryonic stem cells lacking clathrin-mediated endocytosis cannot be rescued by restoring cellular stiffness.The Journal of biological chemistry · 2020Article
- Emerging roles of cytoskeletal proteins in regulating gene expression and genome organization during differentiation.Nucleus (Austin, Tex.) · 2020Review
- Article
- Actomyosin and the MRTF-SRF pathway downregulate FGFR1 in mesenchymal stromal cells.Communications biology · 2020Article
- ROCK inhibitor combined with CaScientific reports · 2020Article
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
Use of stem cell-based therapies in tissue engineering and regenerative medicine is hindered by efficient means of directed differentiation. For pluripotent stem cells, an initial critical differentiation event is specification to one of three germ lineages: endoderm, mesoderm, and ectoderm. Differentiation is known to be regulated by numerous extracellular and intracellular factors, but the role of the cytoskeleton during specification, or early differentiation, is still unknown. In these studies, we used agonists and antagonists to modulate actin polymerization and the actin-myosin molecular motor during spontaneous differentiation of embryonic stem cells in embryoid bodies. We found that inhibiting either actin polymerization or actin-myosin interactions led to a decrease in differentiation to the mesodermal lineage and an increase in differentiation to the endodermal lineage. Thus, targeting processes that regulate cytoskeletal tension may be effective in enhancing or inhibiting differentiation towards cells of the endodermal or mesodermal lineages, which include hepatocytes, islets, cardiomyocytes, endothelial cells, and osteocytes. Therefore, these fundamental findings demonstrate that modulation of the cytoskeleton may be useful in production for a range of cell-based therapies, including for liver, pancreatic, cardiac, vascular, and orthopedic applications.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.