ArticleHistochemistry and cell biology2018
Ultra-structure of the sperm head-to-tail linkage complex in the absence of the spermatid-specific LINC component SPAG4.
Article in Histochemistry and cell biology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed.
- TENT5C extends Odf1 poly(A) tail to sustain sperm morphogenesis and fertility.Nature communications · 2026Article
- Togaram Ensures Axial Alignment of the Sperm Neck.bioRxiv : the preprint server for biology · 2026Article
- SUN5 forms a regular protein lattice reinforcing the sperm head-tail junction.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- The spermatozoon neck role in infertility and intracytoplasmic sperm injection outcomes.Journal of assisted reproduction and genetics · 2026Review
- Molecular models of the sperm head-tail coupling apparatus.Journal of cell science · 2025Review
- Function of manchette and intra-manchette transport in spermatogenesis and male fertility.Cell communication and signaling : CCS · 2025Review
- Comparative Genomics Supports Ecologically Induced Selection as a Putative Driver of Banded Penguin Diversification.Molecular biology and evolution · 2024Article
- SUN4 is a spermatid type II inner nuclear membrane protein that forms heteromeric assemblies with SUN3 and interacts with lamin B3.Journal of cell science · 2023Article
- Article
- Development of the Connecting Piece in ODF1-Deficient Mouse Spermatids.International journal of molecular sciences · 2022Article
- Sperm bauplan and function and underlying processes of sperm formation and selection.Physiological reviews · 2022Review
- Sperm Associated Antigens: Vigorous Influencers in Life.Cell journal · 2021Review
- Review
- Review
- Identification and Characterization of the Most Common Genetic Variant Responsible for Acephalic Spermatozoa Syndrome in Men Originating from North Africa.International journal of molecular sciences · 2021Article
- SUN5 Interacting With Nesprin3 Plays an Essential Role in Sperm Head-to-Tail Linkage: Research on Sun5 Gene Knockout Mice.Frontiers in cell and developmental biology · 2021Article
- The Role of the LINC Complex in Sperm Development and Function.International journal of molecular sciences · 2020Review
- The WD40-protein CFAP52/WDR16 is a centrosome/basal body protein and localizes to the manchette and the flagellum in male germ cells.Scientific reports · 2020Article
- Sperm Differentiation: The Role of Trafficking of Proteins.International journal of molecular sciences · 2020Review
- Sperm Head-Tail Linkage Requires Restriction of Pericentriolar Material to the Proximal Centriole End.Developmental cell · 2020Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
Tight connection between sperm head and tail is crucial for the transport of the male genome and fertilization. The linkage complex, the sperm head-to-tail coupling apparatus (HTCA), originates from the centrosome and anchors to the nuclear membrane. In contrast to its ultra-structural organization, which is already well known for decades, its protein composition largely still awaits future deciphering. SUN-domain proteins are essential components of a complex that links the cytoskeleton to the peripheral nucleoskeleton, which is the nuclear lamina. Here, we studied the impact of the SUN protein SPAG4/SUN4 on the formation of the HTCA. SPAG4/SUN4 is specifically expressed in haploid male germ cells showing a polarized distribution towards the posterior pole in late spermatids that corresponds to the tail attachment site. SPAG4-deficient male mice are infertile with compromised manchette formation and malformed sperm heads. Nonetheless, sperm tails are present demonstrating dispensability of a proper manchette for their formation. Ultra-structural analyses revealed that the development of the sperm head-to-tail linkage complex in the absence of SPAG4 resembles that in the wild type. However, in SPAG4-deficient sperm, the attachment site is diminished with obvious lateral detachment of the HTCA from the nucleus. Our results thus indicate that SPAG4, albeit not essential for the formation of the HTCA per se, is, nevertheless, required for tightening the sperm head-to-tail anchorage by provoking the correct attachment of the lateral parts of the basal plate to the implantation fossa.
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