Evidence map›Paper›PMID 29663061›Full record

ArticleAnalytical and bioanalytical chemistry2018

Sizing lipid droplets from adult and geriatric mouse liver tissue via nanoparticle tracking analysis.

Katherine A Muratore, Charles P Najt, Nicholas M Livezey, James Marti, Douglas G Mashek, Edgar A Arriaga

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Analytical and bioanalytical chemistry, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed.

  1. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Katherine A MuratoreDepartment of Biochemistry, Molecular Biology, and Biophysics, University of Minnesota, 321 Church Street SE, Minneapolis, MN, 55455, USA.
Charles P NajtDepartment of Biochemistry, Molecular Biology, and Biophysics, University of Minnesota, 321 Church Street SE, Minneapolis, MN, 55455, USA.
Nicholas M LivezeyDepartment of Chemistry, University of Minnesota, 207 Pleasant St SE, Minneapolis, MN, 55455, USA.
James MartiMinnesota Nano Center, University of Minnesota, 115 Union St. SE, Minneapolis, MN, 55455, USA.
Douglas G MashekDepartment of Biochemistry, Molecular Biology, and Biophysics, University of Minnesota, 321 Church Street SE, Minneapolis, MN, 55455, USA.
Edgar A ArriagaDepartment of Biochemistry, Molecular Biology, and Biophysics, University of Minnesota, 321 Church Street SE, Minneapolis, MN, 55455, USA. arriaga@umn.edu.

Funding

Training Grant: Functional Proteomics of AgingT32AG029796 · NIA · UNIVERSITY OF MINNESOTA · PI EDGAR A ARRIAGA, Douglas G Mashek · 2008 to 2026
$6.6M
Quantitating Mitophagy in Skeletal Muscle ModelsR01AG020866 · NIA · UNIVERSITY OF MINNESOTA TWIN CITIES · PI ARRIAGA, EDGAR A · 2002 to 2021
$5.7M
TRAINING GRANT IN DIABETES, ENDOCRINOLOGY, &METABOLISMT32DK007203 · NIDDK · UNIVERSITY OF MINNESOTA TWIN CITIES · PI LISA Senye CHOW, Douglas G Mashek · 1986 to 2026
$4.3M
Chemistry-Biology Interface Training GrantT32GM008700 · NIGMS · UNIVERSITY OF MINNESOTA TWIN CITIES · PI DISTEFANO, MARK D · 1999 to 2018
$4.0M
Regulation and consequences of hepatic lipid droplet catabolismR01DK114401 · NIDDK · UNIVERSITY OF MINNESOTA · PI MASHEK, DOUGLAS G · 2017 to 2020
$1.7M
Role of ATGL and lipid metabolism in healthspanR01AG055452 · NIA · UNIVERSITY OF MINNESOTA · PI MASHEK, DOUGLAS G · 2017 to 2021
$1.6M
National Institute on Aging AG029796National Institutes of Health GM008700NIA NIH HHS R01 AG020866NIA NIH HHS R01 AG055452NIA NIH HHS T32 AG029796NIDDK NIH HHS R01 DK114401NIDDK NIH HHS T32 DK007203NIGMS NIH HHS T32 GM008700
6 · The paper itself

Abstract

The significance of lipid droplets in lipid metabolism, cell signaling, and regulating longevity is increasingly recognized, yet the lipid droplet's unique properties and architecture make it difficult to size and study using conventional methods. To begin to address this issue, we demonstrate the capabilities of nanoparticle tracking analysis (NTA) for sizing of lipid droplets. NTA was found to be adequate to assess lipid droplet stability over time, indicating that lipid droplet preparations are stable for up to 24 h. NTA had the ability to compare the size distributions of lipid droplets from adult and geriatric mouse liver tissue, suggesting an age-related decrease in lipid droplet size. This is the first report on the use of NTA to size intracellular organelles. Graphical Abstract Light scattering reveals the temporal positions of individual lipid droplets, which are recorded with a camera. The two-dimensional diffusion constant of each lipid droplet is extracted from the data set, which is then used to calculate a hydrodynamic radius using the Stokes-Einstein equation.

Indexed as

AgingAnimalsDiffusionDynamic Light ScatteringFemaleLipid DropletsLiverMiceMice, Inbred C57BLNanoparticlesParticle SizeAgingLipid dropletsLiverNanoparticle tracking analysisOrganelles

Identifiers

PMID29663061
PMCPMC5955843

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.