ArticleBioMed research international2018
Revealing the Amylase Interactome in Whole Saliva Using Proteomic Approaches.
Article in BioMed research international, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 33 citations in OpenAlex.
- Salivary Proteome Insights: Evaluation of Saliva Preparation Methods in Mucopolysaccharidoses Research.Biomedicines · 2025Article
- Current approaches to produce durable biomaterials: Trends in polymeric materials for restorative dentistry applications.Dental materials : official publication of the Academy of Dental Materials · 2024Review
- Role of Food Texture, Oral Processing Responses, Bolus Properties, and Digestive Conditions on the Nutrient Bioaccessibility of Al Dente and Soft-Cooked Red Lentil Pasta.Foods (Basel, Switzerland) · 2024Article
- Enhancing saliva diagnostics: The impact of amylase depletion on MALDI-ToF MS profiles as applied to COVID-19.Journal of mass spectrometry and advances in the clinical lab · 2024Article
- Article
- Potential Roles of α-amylase in Alzheimer's Disease: Biomarker and Drug Target.Current neuropharmacology · 2022Review
- The role of mechanical control of biofilm in the salivary pH after sucrose exposure in children with early childhood caries.Scientific reports · 2021Article
- Evaluating protein binding specificity of titanium surfaces through mass spectrometry-based proteomics.Clinical oral investigations · 2021Article
- Differentially Expressed Salivary Proteins in Dental Caries Patients.BioMed research international · 2021Article
- Proteome difference among the salivary proteins adsorbed onto metallic orthodontic brackets and hydroxyapatite discs.PloS one · 2021Article
- Murine Salivary Amylase Protects AgainstFrontiers in physiology · 2021Article
- Quantify the Protein-Protein Interaction Effects on Adsorption Related Lubricating Behaviors of α-Amylase on a Glass Surface.Polymers · 2020Article
- Restoration of Cyclo-Gly-Pro-induced salivary hyposecretion and submandibular composition by naloxone in mice.PloS one · 2020Article
- Protein-Based Salivary Profiles as Novel Biomarkers for Oral Diseases.Disease markers · 2018Review
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Understanding proteins present in saliva and their function when isolated is not enough to describe their real role in the mouth. Due to protein-protein interactions, structural changes may occur in macromolecules leading to functional modulation or modification. Besides amylase's function in carbohydrate breakdown, amylase can delay proteolytic degradation of protein partners (e.g., histatin 1) when complexed. Due to its biochemical characteristics and high abundance in saliva, amylase probably interacts with several proteins acting as a biological carrier. This study focused on identifying interactions between amylase and other proteins found in whole saliva (WS) using proteomic approaches. Affinity chromatography was used, followed by gel electrophoresis methods, sodium dodecyl sulfate and native, tryptic in-solution and in-gel digestion, and mass spectrometry. We identified 66 proteins that interact with amylase in WS. Characterization of the identified proteins suggests that acidic (pI < 6.8) and low molecular weight (MW < 56 kDa) proteins have preference during amylase complex formation. Most of the identified proteins present biological functions related to host protection. A new protein-amylase network was constructed using the STRING database. Further studies are necessary to investigate individualities of the identified amylase interactors. These observations open avenues for more comprehensive studies on not yet fully characterized biological function of amylase.
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