ArticleJournal of circulating biomarkers
Strategies for enumeration of circulating microvesicles on a conventional flow cytometer: Counting beads and scatter parameters.
Article in Journal of circulating biomarkers. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
10 citing papers in PubMed.
- SLAMF7 regulates goblet cell mucus production and negatively impacts gut homeostasis and commensalism.Gut microbes · 2025Article
- Circulating Exosomes and Ambient Air Pollution Exposure in COPD.Chronic obstructive pulmonary diseases (Miami, Fla.) · 2023Article
- Physiological homeostasis alteration and cellular structure damage of Chlorella vulgaris exposed to silver nanoparticles with various microstructural morphologies.Environmental science and pollution research international · 2022Article
- Photobiomodulation induces microvesicle release in human keratinocytes: PI3 kinase-dependent pathway role.Lasers in medical science · 2022Article
- Archaeal extracellular vesicles are produced in an ESCRT-dependent manner and promote gene transfer and nutrient cycling in extreme environments.The ISME journal · 2021Article
- Effects of allelochemical artemisinin in Artemisia annua on Microcystis aeruginosa: growth, death mode, and microcystin-LR changes.Environmental science and pollution research international · 2021Article
- Ivabradine-Stimulated Microvesicle Release Induces Cardiac Protection against Acute Myocardial Infarction.International journal of molecular sciences · 2020Article
- Endothelial Microparticle as an early Marker of Endothelial Dysfunction in Patients with Essential Hypertension: A Pilot Study.Indian journal of clinical biochemistry : IJCB · 2020Article
- Microvesicles from indoxyl sulfate-treated endothelial cells induce vascular calcificationComputational and structural biotechnology journal · 2020Article
- Lab-on-Chip for Exosomes and Microvesicles Detection and Characterization.Sensors (Basel, Switzerland) · 2018Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Enumeration of circulating microvesicles (MVs) by conventional flow cytometry is accomplished by the addition of a known amount of counting beads and calculated from the formula: MV/μl = (MV count/bead count) × final bead concentration. We sought to optimize each variable in the equation by determining the best parameters for detecting 'MV count' and examining the effects of different bead preparations and concentrations on the final calculation. Three commercially available bead preparations (TruCount, Flow-Count and CountBright) were tested, and MV detection on a BD FACSCanto was optimized for gating by either forward scatter (FSC) or side scatter (SSC); the results were compared by calculating different subsets of MV on a series of 74 typical patient plasma samples. The relationship between the number of beads added to each test and the number of beads counted by flow cytometry remained linear over a wide range of bead concentrations (
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.