ArticleScientific reports2018
Optical interrogation of neuronal circuitry in zebrafish using genetically encoded voltage indicators.
Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 27 citations in OpenAlex.
- Review
- Voltage imaging in zebrafish using high-speed light-sheet microscopy.Neurophotonics · 2026Review
- CaMPARI2 enables stimulus-locked whole-brain activity mapping at cellular resolution in unrestrained larval zebrafish.Frontiers in molecular neuroscience · 2026Article
- CaMPARI2 Enables Stimulus-Locked Whole-Brain Activity Mapping at Cellular Resolution in Unrestrained Larval Zebrafish.bioRxiv : the preprint server for biology · 2025Article
- Review
- Article
- Zebrafish behavior as a gateway to nervous system assembly and plasticity.Development (Cambridge, England) · 2022Article
- Calcium Signaling in the Cerebellar Radial Glia and Its Association with Morphological Changes during Zebrafish Development.International journal of molecular sciences · 2021Article
- Two-Photon Laser Ablation and In Vivo Wide-Field Imaging of Inferior Olive Neurons Revealed the Recovery of Olivocerebellar Circuits in Zebrafish.International journal of environmental research and public health · 2021Article
- Drug Screening with Genetically Encoded Fluorescent Sensors: Today and Tomorrow.International journal of molecular sciences · 2020Review
- Genetically Encoded Tools for Research of Cell Signaling and Metabolism under Brain Hypoxia.Antioxidants (Basel, Switzerland) · 2020Review
- NOSA, an Analytical Toolbox for Multicellular Optical Electrophysiology.Frontiers in neuroscience · 2020Article
- Modeling Neuronal Diseases in Zebrafish in the Era of CRISPR.Current neuropharmacology · 2020Review
- Optical voltage imaging in neurons: moving from technology development to practical tool.Nature reviews. Neuroscience · 2019Review
- Imaging spinal cord activity in behaving animals.Experimental neurology · 2019Review
Corrections and comments
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Authors and funding
9 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Optical measurement of membrane potentials enables fast, direct and simultaneous detection of membrane potentials from a population of neurons, providing a desirable approach for functional analysis of neuronal circuits. Here, we applied recently developed genetically encoded voltage indicators, ASAP1 (Accelerated Sensor of Action Potentials 1) and QuasAr2 (Quality superior to Arch 2), to zebrafish, an ideal model system for studying neurogenesis. To achieve this, we established transgenic lines which express the voltage sensors, and showed that ASAP1 is expressed in zebrafish neurons. To examine whether neuronal activity could be detected by ASAP1, we performed whole-cerebellum imaging, showing that depolarization was detected widely in the cerebellum and optic tectum upon electrical stimulation. Spontaneous activity in the spinal cord was also detected by ASAP1 imaging at single-cell resolution as well as at the neuronal population level. These responses mostly disappeared following treatment with tetrodotoxin, indicating that ASAP1 enabled optical measurement of neuronal activity in the zebrafish brain. Combining this method with other approaches, such as optogenetics and behavioural analysis may facilitate a deeper understanding of the functional organization of brain circuitry and its development.
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