ArticleInternational journal of molecular sciences2018
Nitrate Reductases Are Relocalized to the Nucleus by AtSIZ1 and Their Levels Are Negatively Regulated by COP1 and Ammonium.
Article in International journal of molecular sciences, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 23 citations in OpenAlex.
- Insects manipulate host nuclear trafficking and epigenetic regulation to facilitate herbivory.Nature communications · 2026Article
- Highly sensitive site-specific SUMOylation proteomics in Arabidopsis.Nature plants · 2024Article
- PYRIDOX(AM)INE 5'-PHOSPHATE OXIDASE3 of Arabidopsis thaliana maintains carbon/nitrogen balance in distinct environmental conditions.Plant physiology · 2023Article
- The Regulation of Nitrate Reductases in Response to Abiotic Stress in Arabidopsis.International journal of molecular sciences · 2022Article
- Protein Tyrosine Nitration in Plant Nitric Oxide Signaling.Frontiers in plant science · 2022Review
- Current perspectives of ubiquitination and SUMOylation in abiotic stress tolerance in plants.Frontiers in plant science · 2022Review
- The SUMO E3 Ligase MdSIZ1 Sumoylates a Cell Number Regulator MdCNR8 to Control Organ Size.Frontiers in plant science · 2022Article
- Phytosulfokine α (PSKα) delays senescence and reinforces SUMO1/SUMO E3 ligase SIZ1 signaling pathway in cut rose flowers (Rosa hybrida cv. Angelina).Scientific reports · 2021Article
- Ammonium-mediated reduction in salicylic acid content and recovery of plant growth in ArabidopsisPlant signaling & behavior · 2021Article
- Lessons from Comparison of Hypoxia Signaling in Plants and Mammals.Plants (Basel, Switzerland) · 2021Review
- Post-Translational Modifications of Nitrate Reductases Autoregulates Nitric Oxide Biosynthesis in Arabidopsis.International journal of molecular sciences · 2021Article
- Nitrogen and Stem Development: A Puzzle Still to Be Solved.Frontiers in plant science · 2021Review
- Submergence and Waterlogging Stress in Plants: A Review Highlighting Research Opportunities and Understudied Aspects.Frontiers in plant science · 2019Review
- Protein Phosphatase (International journal of molecular sciences · 2018Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Nitrate reductases (NRs) catalyze the first step in the reduction of nitrate to ammonium. NR activity is regulated by sumoylation through the E3 ligase activity of AtSIZ1. However, it is not clear how NRs interact with AtSIZ1 in the cell, or how nitrogen sources affect NR levels and their cellular localization. Here, we show that the subcellular localization of NRs is modulated by the E3 SUMO (Small ubiquitin-related modifier) ligase AtSIZ1 and that NR protein levels are regulated by nitrogen sources. Transient expression analysis of GFP fusion proteins in onion epidermal cells showed that the NRs NIA1 and NIA2 localize to the cytoplasmic membrane, and that AtSIZ1 localizes to the nucleoplasm, including nuclear bodies, when expressed separately, whereas NRs and AtSIZ1 localize to the nucleus when co-expressed. Nitrate did not affect the subcellular localization of the NRs, but it caused AtSIZ1 to move from the nucleus to the cytoplasm. NRs were not detected in ammonium-treated cells, whereas the localization of AtSIZ1 was not altered by ammonium treatment. NR protein levels increased in response to nitrate but decreased in response to ammonium. In addition, NR protein levels increased in response to a 26S proteasome inhibitor and in
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