ArticleNucleic acids research2018
Translatome analysis at the egg-to-embryo transition in sea urchin.
Article in Nucleic acids research, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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12 citing papers in PubMed, 26 citations in OpenAlex.
- Spatiotemporal requirements of nuclear β-catenin define early sea urchin embryogenesis.PLoS biology · 2024Article
- The dynamics and functional impact of tRNA repertoires during early embryogenesis in zebrafish.The EMBO journal · 2024Article
- Stable germline transgenesis using the Minos Tc1/mariner element in the sea urchin Lytechinus pictus.Development (Cambridge, England) · 2024Article
- eIF4E1b is a non-canonical eIF4E protecting maternal dormant mRNAs.EMBO reports · 2024Article
- Article
- Evolution of the Neocortex Through RNA-Binding Proteins and Post-transcriptional Regulation.Frontiers in neuroscience · 2021Review
- Identifying the Translatome of Mouse NEBD-Stage Oocytes via SSP-Profiling; A Novel Polysome Fractionation Method.International journal of molecular sciences · 2020Article
- Induced androgenetic development in rainbow trout and transcriptome analysis of irradiated eggs.Scientific reports · 2019Article
- A Yeast System for Discovering Optogenetic Inhibitors of Eukaryotic Translation Initiation.ACS synthetic biology · 2019Article
- Translational Control of Canonical and Non-Canonical Translation Initiation Factors at the Sea Urchin Egg to Embryo Transition.International journal of molecular sciences · 2019Article
- Trapping, tagging and tracking: Tools for the study of proteins during early development of the sea urchin.Methods in cell biology · 2019Review
- Multiplexing polysome profiling experiments to study translation in Escherichia coli.PloS one · 2019Article
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Early embryogenesis relies on the translational regulation of maternally stored mRNAs. In sea urchin, fertilization triggers a dramatic rise in translation activity, necessary for the onset of cell division. Here, the full spectrum of the mRNAs translated upon fertilization was investigated by polysome profiling and sequencing. The translatome of the early sea urchin embryo gave a complete picture of the polysomal recruitment dynamics following fertilization. Our results indicate that only a subset of maternal mRNAs were selectively recruited onto polysomes, with over-represented functional categories in the translated set. The increase in translation upon fertilization depends on the formation of translation initiation complexes following mTOR pathway activation. Surprisingly, mTOR pathway inhibition differentially affected polysomal recruitment of the newly translated mRNAs, which thus appeared either mTOR-dependent or mTOR-independent. Therefore, our data argue for an alternative to the classical cap-dependent model of translation in early development. The identification of the mRNAs translated following fertilization helped assign translational activation events to specific mRNAs. This translatome is the first step to a comprehensive analysis of the molecular mechanisms governing translation upon fertilization and the translational regulatory networks that control the egg-to-embryo transition as well as the early steps of embryogenesis.
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