ArticleMolecular biology and evolution2018
Synergistic Binding of bHLH Transcription Factors to the Promoter of the Maize NADP-ME Gene Used in C4 Photosynthesis Is Based on an Ancient Code Found in the Ancestral C3 State.
Article in Molecular biology and evolution, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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23 citing papers in PubMed, 47 citations in OpenAlex.
- Review
- A single-cell rice atlas integrates multi-species data to reveal cis-regulatory evolution.Nature plants · 2025Article
- Construction of cDNA library of Dalbergia odorifera induced by low temperature stress and screening of low temperature tolerant genes.PloS one · 2025Article
- Molecular Mechanisms Underlying Freezing Tolerance in Plants: Implications for Cryopreservation.International journal of molecular sciences · 2024Review
- Screening and Validation of Leaf Width-Related Genes in Inbred Maize Lines.Life (Basel, Switzerland) · 2024Article
- Cucumber malate decarboxylase, CsNADP-ME2, functions in the balance of carbon and amino acid metabolism in fruit.Horticulture research · 2023Article
- Compartmentation of photosynthesis gene expression in C4 maize depends on time of day.Plant physiology · 2023Article
- Epi-Brassinolide RegulatesInternational journal of molecular sciences · 2023Article
- Overexpression of cytoplasmic CPlant biotechnology journal · 2022Article
- Wheat-Frontiers in plant science · 2022Article
- Network and epigenetic characterization of subsets of genes specifically expressed in maize bundle sheath cells.Computational and structural biotechnology journal · 2022Article
- Unraveling the malate biosynthesis during development ofCurrent research in food science · 2022Article
- Insights into Regulation of CInternational journal of molecular sciences · 2021Article
- ZmOrphan94 Transcription Factor DownregulatesFrontiers in plant science · 2021Article
- Genetic Diversity of CGenes · 2020Article
- Continued Adaptation of C4 Photosynthesis After an Initial Burst of Changes in the Andropogoneae Grasses.Systematic biology · 2020Article
- Physiological and Molecular Mechanism Involved in Cold Stress Tolerance in Plants.Plants (Basel, Switzerland) · 2020Review
- Insights Into the Regulation of the Expression Pattern of Calvin-Benson-Bassham Cycle Enzymes in CFrontiers in plant science · 2020Article
- Genome-Wide Transcription Factor Binding in Leaves from CThe Plant cell · 2019Article
- The genetics of convergent evolution: insights from plant photosynthesis.Nature reviews. Genetics · 2019Review
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Authors and funding
12 authors at 3 institutions in 2 countries.
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Abstract
C4 photosynthesis has evolved repeatedly from the ancestral C3 state to generate a carbon concentrating mechanism that increases photosynthetic efficiency. This specialized form of photosynthesis is particularly common in the PACMAD clade of grasses, and is used by many of the world's most productive crops. The C4 cycle is accomplished through cell-type-specific accumulation of enzymes but cis-elements and transcription factors controlling C4 photosynthesis remain largely unknown. Using the NADP-Malic Enzyme (NADP-ME) gene as a model we tested whether mechanisms impacting on transcription in C4 plants evolved from ancestral components found in C3 species. Two basic Helix-Loop-Helix (bHLH) transcription factors, ZmbHLH128 and ZmbHLH129, were shown to bind the C4NADP-ME promoter from maize. These proteins form heterodimers and ZmbHLH129 impairs trans-activation by ZmbHLH128. Electrophoretic mobility shift assays indicate that a pair of cis-elements separated by a seven base pair spacer synergistically bind either ZmbHLH128 or ZmbHLH129. This pair of cis-elements is found in both C3 and C4 Panicoid grass species of the PACMAD clade. Our analysis is consistent with this cis-element pair originating from a single motif present in the ancestral C3 state. We conclude that C4 photosynthesis has co-opted an ancient C3 regulatory code built on G-box recognition by bHLH to regulate the NADP-ME gene. More broadly, our findings also contribute to the understanding of gene regulatory networks controlling C4 photosynthesis.
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