ArticleRNA biology2018
Live-cell imaging reveals the dynamics and function of single-telomere TERRA molecules in cancer cells.
Article in RNA biology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed.
- Sensitivity Enhancement of Dynamic Full-Field Optical Coherence Tomography Using Ratio-Free Detection and Partial-Field Illumination for Retinal Organoid Imaging.Bioengineering (Basel, Switzerland) · 2026Article
- Beyond the ends: potential implications of telomeric repeat-containing RNA (TERRA) for CNS diseases.Journal of molecular cell biology · 2025Review
- Delayed complications of sulfur mustard poisoning: a focus on inflammation and telomere footprint.Archives of toxicology · 2025Review
- TERRA transcripts localize at long telomeres to regulate telomerase access to chromosome ends.Science advances · 2024Article
- TERRA-LSD1 phase separation promotes R-loop formation for telomere maintenance in ALT cancer cells.Nature communications · 2024Article
- TERRA expression is regulated by the telomere-binding proteins POT-1 and POT-2 in Caenorhabditis elegans.Nucleic acids research · 2023Article
- Dynamic full-field optical coherence tomography module adapted to commercial microscopes allows longitudinal in vitro cell culture study.Communications biology · 2023Article
- 5-HTInternational journal of molecular sciences · 2023Article
- Review
- A guide for single-particle chromatin tracking in live cell nuclei.Cell biology international · 2022Review
- HOTAIR beyond repression: In protein degradation, inflammation, DNA damage response, and cell signaling.DNA repair · 2021Review
- Article
- TERRA transcription destabilizes telomere integrity to initiate break-induced replication in human ALT cells.Nature communications · 2021Article
- CRISPR Cas13-Based Tools to Track and Manipulate Endogenous Telomeric Repeat-Containing RNAs in Live Cells.Frontiers in molecular biosciences · 2021Article
- A Tale of Two States: Pluripotency Regulation of Telomeres.Frontiers in cell and developmental biology · 2021Review
- Imaging mRNA trafficking in living cells using fluorogenic proteins.Current opinion in chemical biology · 2020Review
- Noncoding telomeric repeat-containing RNA inhibits the progression of hepatocellular carcinoma by regulating telomerase-mediated telomere length.Cancer science · 2020Article
- Imaging of DNA and RNA in Living Eukaryotic Cells to Reveal Spatiotemporal Dynamics of Gene Expression.Annual review of biochemistry · 2020Review
- Review
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
Telomeres cap the ends of eukaryotic chromosomes, protecting them from degradation and erroneous recombination events which may lead to genome instability. Telomeres are transcribed giving rise to telomeric repeat-containing RNAs, called TERRA. The TERRA long noncoding RNAs have been proposed to play important roles in telomere biology, including heterochromatin formation and telomere length homeostasis. While TERRA RNAs are predominantly nuclear and localize at telomeres, little is known about the dynamics and function of TERRA molecules expressed from individual telomeres. Herein, we developed an assay to image endogenous TERRA molecules expressed from a single telomere in living human cancer cells. We show that single-telomere TERRA can be detected as TERRA RNA single particles which freely diffuse within the nucleus. Furthermore, TERRA molecules aggregate forming TERRA clusters. Three-dimensional size distribution and single particle tracking analyses revealed distinct sizes and dynamics for TERRA RNA single particles and clusters. Simultaneous time lapse confocal imaging of TERRA particles and telomeres showed that TERRA clusters transiently co-localize with telomeres. Finally, we used chemically modified antisense oligonucleotides to deplete TERRA molecules expressed from a single telomere. Single-telomere TERRA depletion resulted in increased DNA damage at telomeres and elsewhere in the genome. These results suggest that single-telomere TERRA transcripts participate in the maintenance of genomic integrity in human cancer cells.
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Registered trials
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