ArticleNano letters2018
Quantifying Reversible Surface Binding via Surface-Integrated Fluorescence Correlation Spectroscopy.
Article in Nano letters, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
What it found
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
12 citing papers in PubMed.
- Fluorescence Correlation Spectroscopy to Examine Protein-Lipid Interactions in Membranes.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Correction of Background in Fluorescence Correlation Spectroscopy for Accurate Determination of Particle Number.Biomolecules · 2025Article
- Bayesian Inference of Binding Kinetics from Fluorescence Time Series.The journal of physical chemistry. B · 2025Article
- Functionalizing DNA origami to investigate and interact with biological systems.Nature reviews. Materials · 2023Article
- Precision and Accuracy of Receptor Quantification on Synthetic and Biological Surfaces Using DNA-PAINT.ACS sensors · 2023Article
- Calibration-free counting of low molecular copy numbers in single DNA-PAINT localization clusters.Biophysical reports · 2021Article
- Article
- Toward Absolute Molecular Numbers in DNA-PAINT.Nano letters · 2019Article
- Flat-top TIRF illumination boosts DNA-PAINT imaging and quantification.Nature communications · 2019Article
- Review
- Photo-Induced Depletion of Binding Sites in DNA-PAINT Microscopy.Molecules (Basel, Switzerland) · 2018Article
- The MinDE system is a generic spatial cue for membrane protein distribution in vitro.Nature communications · 2018Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We present a simple and versatile single-molecule-based method for the accurate determination of binding rates to surfaces or surface bound receptors. To quantify the reversible surface attachment of fluorescently labeled molecules, we have modified previous schemes for fluorescence correlation spectroscopy with total internal reflection illumination (TIR-FCS) and camera-based detection. In contrast to most modern applications of TIR-FCS, we completely disregard spatial information in the lateral direction. Instead, we perform correlation analysis on a spatially integrated signal, effectively converting the illuminated surface area into the measurement volume. In addition to providing a high surface selectivity, our new approach resolves association and dissociation rates in equilibrium over a wide range of time scales. We chose the transient hybridization of fluorescently labeled single-stranded DNA to the complementary handles of surface-immobilized DNA origami structures as a reliable and well-characterized test system. We varied the number of base pairs in the duplex, yielding different binding times in the range of hundreds of milliseconds to tens of seconds, allowing us to quantify the respective surface affinities and binding rates.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.