Evidence map›Paper›PMID 29657902›Full record

ArticleTranslational vision science & technology2018

Inhibitory Effects of Angiotensin II Receptor Blockade on Human Tenon Fibroblast Migration and Reactive Oxygen Species Production in Cell Culture.

Duri Kim, Ushasree Pattamatta, Elizabeth Kelly, Paul R Healey, Nicole Carnt, Hans Zoellner, Andrew J R White

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Translational vision science & technology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.5field-weighted citation impact, top 37% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 9 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 3 institutions in 1 country.

Duri KimCentre for Vision Research, the Westmead Institute for Medical Research, Sydney Medical School, University of Sydney, New South Wales, Australia.
Ushasree PattamattaCentre for Vision Research, the Westmead Institute for Medical Research, Sydney Medical School, University of Sydney, New South Wales, Australia.
Elizabeth KellyThe Cellular and Molecular Pathology Research Unit, Department of Oral Pathology, Faculty of Dentistry, University of Sydney, Westmead Hospital, New South Wales, Australia.
Paul R HealeyCentre for Vision Research, the Westmead Institute for Medical Research, Sydney Medical School, University of Sydney, New South Wales, Australia.
Nicole CarntCentre for Vision Research, the Westmead Institute for Medical Research, Sydney Medical School, University of Sydney, New South Wales, Australia.
Hans ZoellnerThe Cellular and Molecular Pathology Research Unit, Department of Oral Pathology, Faculty of Dentistry, University of Sydney, Westmead Hospital, New South Wales, Australia.
Andrew J R WhiteCentre for Vision Research, the Westmead Institute for Medical Research, Sydney Medical School, University of Sydney, New South Wales, Australia.
The University of Sydney · AUWestmead Hospital · AUWestmead Institute for Medical Research · AU

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

purposeWe investigate the effect of angiotensin receptor blockade on the migration of human Tenon fibroblasts (HTF), using irbesartan, an angiotensin II receptor type 1 (AT1R) blocker (ARB) as a potential antifibrotic agent in glaucoma filtration surgery.

methodsConfluent HTF cultures were scratched with a 1 mL pipette tip and treated with either irbesartan (10, 50, and 100 μg/mL) or angiotensin II (2 μg/mL). The extent of HTF migration up to 30 hours, and cell number and morphology at 72 hours was evaluated. To assess the effect on reactive oxygen species (ROS) level, HTF were treated with either irbesartan (10 μg/mL) or angiotensin II (2 μg/mL) for 24 hours after scratching, and then stained with dihydroethidium (DHE) before evaluation by confocal microscopy.

resultsIrbesartan inhibited HTF migration by 50% to 70% compared to controls (

conclusionsIrbesartan inhibited HTF migration and ROS production. It also reduced cell numbers and altered HTF morphology. Angiotensin II increased cell number without altering morphology. This initial study warrants future investigations for further potential antifibrotic effects of this drug. TRANSLATIONAL RELEVANCE: This in vitro study focused on investigations of irbesartan's effects on HTF migration, ROS production, as well as HTF cell numbers and morphology. It suggests a potential therapeutic strategy worth further exploration with a view towards postoperative wound healing modulation in glaucoma filtration surgery.

Indexed as

angiotensin IIcell migrationglaucomahuman Tenon's fibroblastwound healing

Identifiers

PMID29657902
PMCPMC5896341
OpenAlexW2796986305

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.