ArticleVeterinary world2018
DNA extraction from hydatid cyst protoscolices: Comparison of five different methods.
Article in Veterinary world, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed.
- Efficient DNA Extraction Coupled with Fluorescence Loop-Mediated Isothermal Amplification (LAMP) for On-Site Early Warning Detection of Vibrio parahaemolyticus.Marine biotechnology (New York, N.Y.) · 2026Article
- Molecular evidence of Echinococcus canadensis (G6/G7) predominance in Mongolian livestock and its implications for control.PLoS neglected tropical diseases · 2026Article
- Echinococcus granulosus sensu stricto hydatid cysts in human and livestock from northeastern Algeria: Molecular characterization based on nad5 and cox1 and fertility assessment.Parasitology research · 2026Article
- Zwitterionic Poly(sulfobetaine methacrylate) Brushes Functionalized Threads for DNA Extraction from Complex Cell Lysates.Sensors (Basel, Switzerland) · 2025Article
- Update on the genetic diversity and population structure of Echinococcus granulosus in Gansu Province, Tibet Autonomous Region, and Xinjiang Uygur Autonomous Region, Western China, inferred from mitochondrial cox1, nad1, and nad5 sequences.Parasitology research · 2023Article
- Occurrence of microsporidia spp. infection in children with diarrhea in southern Iran.Journal of parasitic diseases : official organ of the Indian Society for Parasitology · 2022Article
- The occurrence of cystic echinococcosis in slaughtered livestock in Jahrom, south of Iran.Parasite epidemiology and control · 2022Article
- Molecular characterization and phylogenetic studies ofOpen life sciences · 2021Article
- Comparison of various methods for DNA extraction from human isolated paraffin-embedded hydatid cyst samples.Journal of parasitic diseases : official organ of the Indian Society for Parasitology · 2020Article
- Echinococcus granulosus (sensu stricto) (G1, G3) and E. ortleppi (G5) in Pakistan: phylogeny, genetic diversity and population structural analysis based on mitochondrial DNA.Parasites & vectors · 2020Article
- Genetic Diversity ofBioMed research international · 2020Article
- Validation of a one-step PCR assay for the molecular identification of Echinococcus granulosus sensu stricto G1-G3 genotype.Molecular biology reports · 2019Article
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4 authors.
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Abstract
aimThe current study aimed to find out a simple, practical and high throughput DNA isolation method for extraction of DNA from hydatid cyst samples. MATERIALS AND
methodsCattle and sheep isolate of hydatid cysts were obtained from the slaughterhouse, and hydatid fluid and protoscolices were collected in a sterile condition. Protoscolices were washed, 3 times with phosphate buffered saline, and DNA was extracted by different methods including manual extraction with freeze/thawing and phenol-chloroform, Triton X-100 extraction, and by a commercial kit (YTA, Yekta Tajhiz Azma, Iran) with three different modifications in the kit's manufacturer instructions. The obtained DNA from the different methods was evaluated by Nanodrop in terms of the yield of DNA and carbohydrates or protein contaminations. To compare the quality of the extracted DNA, two pieces of the mitochondrial genome of
resultsThe DNA extracted by manual method, using phenol/chloroform, had the highest yield, yet with the highest level of protein and carbohydrate contamination. The DNA extracted using two-step incubations, initially at 60°C for 2 h and then overnight at 37°C, was the most purified DNA with the lowest rate of contamination.
conclusionFindings of the study demonstrated that modification in the currently available commercially DNA extraction kit resulted in the development of a high throughput DNA isolation method. This method can be recommended for the extraction of DNA from hydatid cysts, especially the cattle isolate where the extraction of DNA in these samples are usually problematic.
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