ArticleNon-coding RNA2017
Deciphering microRNAs and Their Associated Hairpin Precursors in a Non-Model Plant, Abelmoschus esculentus.
Article in Non-coding RNA, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed.
- Characteristics and Phylogenetic Analysis of the Complete Chloroplast Genome ofInternational journal of molecular sciences · 2025Article
- Integrated stress responses in okra plants (cv. ''Meya']: unravelling the mechanisms underlying drought and nematode co-occurrence.BMC plant biology · 2024Article
- Evidence of microRNAs origination from chloroplast genome and their role in regulating Photosystem II protein N (psbN) mRNA.Biotechnologia · 2024Article
- An Insight Into Pentatricopeptide-Mediated Chloroplast NecrosisFrontiers in genetics · 2022Article
- Genome-wide miRNA analysis and integrated network for flavonoid biosynthesis in Osmanthus fragrans.BMC genomics · 2021Article
- Noncoding RNA: An Insight into Chloroplast and Mitochondrial Gene Expressions.Life (Basel, Switzerland) · 2021Review
- Identification of MicroRNAs inBioMed research international · 2021Article
- Circular RNAs-The Road Less Traveled.Frontiers in molecular biosciences · 2019Review
- Novel and Conserved miRNAs Among Brazilian Pine and Other Gymnosperms.Frontiers in genetics · 2019Article
- Circular RNA Profiling by Illumina Sequencing via Template-Dependent Multiple Displacement Amplification.BioMed research international · 2019Article
- Plant Responses to Pathogen Attack: Small RNAs in Focus.International journal of molecular sciences · 2018Review
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
MicroRNAs (miRNAs) are crucial regulatory RNAs, originated from hairpin precursors. For the past decade, researchers have been focusing extensively on miRNA profiles in various plants. However, there have been few studies on the global profiling of precursor miRNAs (pre-miRNAs), even in model plants. Here, for the first time in a non-model plant-Abelmoschus esculentus with negligible genome information-we are reporting the global profiling to characterize the miRNAs and their associated pre-miRNAs by applying a next generation sequencing approach. Preliminarily, we performed small RNA (sRNA) sequencing with five biological replicates of leaf samples to attain 207,285,863 reads; data analysis using miRPlant revealed 128 known and 845 novel miRNA candidates. With the objective of seizing their associated hairpin precursors, we accomplished pre-miRNA sequencing to attain 83,269,844 reads. The paired end reads are merged and adaptor trimmed, and the resulting 40-241 nt (nucleotide) sequences were picked out for analysis by using perl scripts from the miRGrep tool and an in-house built shell script for Minimum Fold Energy Index (MFEI) calculation. Applying the stringent criteria of the Dicer cleavage pattern and the perfect stem loop structure, precursors for 57 known miRNAs of 15 families and 18 novel miRNAs were revealed. Quantitative Real Time (qRT) PCR was performed to determine the expression of selected miRNAs.
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