ArticleMikrochimica acta2018
Fluorometric aptamer based assay for ochratoxin A based on the use of exonuclease III.
Article in Mikrochimica acta, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 40 citations in OpenAlex.
- In situ generated PANI promoted flexible photoelectrochemical biosensor for ochratoxin A based on GOx-stuffed DNA hydrogel as enhancer.Mikrochimica acta · 2023Article
- Design of a Quencher-Free Fluorescent Aptasensor for Ochratoxin A Detection in Red Wine Based on the Guanine-Quenching Ability.Biosensors · 2022Article
- Nanomaterial-based aptamer biosensors for ochratoxin A detection: a review.Analytical and bioanalytical chemistry · 2022Review
- Amplified Fluorescent Aptasensor for Ochratoxin A Assay Based on Graphene Oxide and RecJToxins · 2020Article
- A fluorometric aptamer-based assay for ochratoxin A by using exonuclease III-assisted recycling amplification.Mikrochimica acta · 2019Article
- A review on recent developments in optical and electrochemical aptamer-based assays for mycotoxins using advanced nanomaterials.Mikrochimica acta · 2019Review
- A chemiluminescence resonance energy transfer strategy and its application for detection of platinum ions and cisplatin.Mikrochimica acta · 2019Article
- A FRET-based dual-color evanescent wave optical fiber aptasensor for simultaneous fluorometric determination of aflatoxin M1 and ochratoxin A.Mikrochimica acta · 2018Article
- SERS based aptasensor for ochratoxin A by combining FeMikrochimica acta · 2018Article
- Aptamer based voltammetric patulin assay based on the use of ZnO nanorods.Mikrochimica acta · 2018Article
- Fluorometric aptamer-based determination of ochratoxin A based on the use of graphene oxide and RNase H-aided amplification.Mikrochimica acta · 2018Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
This study describes an aptamer based assay for the mycotoxin ochratoxin A (OTA). The method is based on the use of an OTA-specific aptamer, exonuclease (Exo) III, SYBR Gold as a fluorescent probe, and a complementary strand that specifically combines with the aptamer. In the presence of OTA, the aptamer and OTA hybridize, thereby resulting in the formation of ssDNA, which is not digested by Exo III. Intense fluorescence is observed after addition of SYBR Gold (best measured at excitation/emission wavelengths of 495/540 nm). Fluorescence increases linearly with the log of the OTA concentration in the range from 8 to 1000 ng·mL
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