Evidence map›Paper›PMID 29654471›Full record

ArticleMolecular biotechnology2018

Haloquadratum walsbyi Yields a Versatile, NAD

Jennifer Cassidy, Francesca Paradisi

Open access · greenAbstract read
PubMed Publisher
In one paragraph

Article in Molecular biotechnology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.1field-weighted citation impact, top 53% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 4 citations in OpenAlex.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors at 1 institution in 2 countries.

Jennifer CassidySchool of Chemistry, University of Nottingham, University Park, NG7 2RD, UK.
Francesca ParadisiSchool of Chemistry, University of Nottingham, University Park, NG7 2RD, UK. Francesca.Paradisi@nottingham.ac.uk.ORCID http://orcid.org/0000-0003-1704-0642
University College Dublin · IE

Funding

Science Foundation Ireland 12/RC/2275
6 · The paper itself

Abstract

This study presents the first example of an alcohol dehydrogenase (ADH) from the halophilic archaeum Haloquadratum walsbyi (HwADH). A hexahistidine-tagged recombinant HwADH was heterologously overexpressed in Haloferax volcanii. HwADH was purified in one step and was found to be thermophilic with optimal activity at 65 °C. HwADH was active in the presence of 10% (v/v) organic solvent. The enzyme displayed dual cofactor specificity and a broad substrate scope, and maximum activity was detected with benzyl alcohol and 2-phenyl-1-propanol. HwADH accepted aromatic ketones, acetophenone and phenylacetone as substrates. The enzyme also accepted cyclohexanol and aromatic secondary alcohols, 1-phenylethanol and 4-phenyl-2-butanol. H. walsbyi may offer an excellent alternative to other archaeal sources to expand the toolbox of halophilic biocatalysts.

Indexed as

Alcohol DehydrogenaseAlcoholsArchaeal ProteinsBenzyl AlcoholCloning, MolecularEnzyme StabilityGenes, ArchaealHalobacteriaceaeHaloferax volcaniiHot TemperatureKineticsNADNADPPropanolsRecombinant ProteinsSubstrate Specificity2-phenylpropanol-1Alcohol DehydrogenaseAlcoholsArchaeal ProteinsBenzyl AlcoholNADNADPPropanolsRecombinant ProteinsAlcohol dehydrogenaseDual cofactor specificityHaloquadratum walsbyiThermoactivity

Identifiers

PMID29654471
OpenAlexW2798076213

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.