ArticleParasites & vectors2018
Effects of antibiotic treatment on the fecundity of Rhipicephalus haemaphysaloides ticks.
Article in Parasites & vectors, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers, 1 of them a synthesis that pooled it.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
28 citing papers in PubMed, 1 synthesis or guideline pooled it, 56 citations in OpenAlex.
- The Prevalence ofFrontiers in veterinary science · 2021Pooled it
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- Evolutionary Convergence of Nutritional Symbionts in Ticks.Environmental microbiology reports · 2025Article
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- Diversity of midgut microbiota in ticks collected from white-tailed deer (Odocoileus virginianus) from northern Mexico.Parasites, hosts and diseases · 2024Article
- Significant role of symbiotic bacteria in the blood digestion and reproduction ofISME communications · 2024Article
- Analysis of the bacterial community in female Rhipicephalus microplus ticks from selected provinces in Luzon, Philippines, using next-generation sequencing.Experimental & applied acarology · 2023Article
- Microbiota in disease-transmitting vectors.Nature reviews. Microbiology · 2023Review
- Investigation of vertical and horizontal transmission of Spiroplasma in ticks under laboratory conditions.Scientific reports · 2023Article
- Review
- Wild Hedgehogs and Their Parasitic Ticks Coinfected with Multiple Tick-Borne Pathogens in Jiangsu Province, Eastern China.Microbiology spectrum · 2022Article
- Ecological Contacts and Host Specificity Promote Replacement of Nutritional Endosymbionts in Ticks.Microbial ecology · 2022Article
- Comparative Proteomics ofMicrobiology spectrum · 2022Article
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- Changes in theFrontiers in microbiology · 2022Article
- Borrelia afzelii Infection in the Rodent Host Has Dramatic Effects on the Bacterial Microbiome of Ixodes ricinus Ticks.Applied and environmental microbiology · 2021Article
- Coxiella burnetii and Related Tick Endosymbionts Evolved from Pathogenic Ancestors.Genome biology and evolution · 2021Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
backgroundEndosymbiotic bacteria inhabit a variety of arthropods including ticks and may have multiple effects on the host's survival, reproduction or pathogen acquisition and transmission. Rhipicephalus haemaphysaloides is one of the most widely distributed tick species in China. The symbiotic bacteria composition and their impacts to R. haemaphysaloides ticks have not been studied. The present study investigated the composition of microbial community in R. haemaphysaloides ticks and then assessed the effects of endosymbionts on the host's fecundity by antibiotic treatment experiments.
methodsThe microbial population of female and male R. haemaphysaloides ticks was analyzed using Illumina Miseq sequencing of 16S rRNA gene. Thirty engorged female ticks were then randomly divided into five groups and injected with ampicillin, ciprofloxacin, kanamycin, tetracycline, or phosphate-buffered solution (PBS), respectively. Effects of antibiotic treatments on maternal oviposition, egg hatching and density of endosymbionts were evaluated.
resultsIllumina Miseq sequencing showed that Coxiella and Rickettsia were the predominant bacterial genera inhabiting R. haemaphysaloides ticks. Antibiotic treatment experiments found that kanamycin reduced the density of Coxiella-like endosymbiont (Coxiella-LE hereafter) in eggs, ciprofloxacin reduced the density of Rickettsia-like endosymbiont (Rickettsia-LE), and tetracycline had effect on both endosymbionts, while ampicillin affected neither. Meanwhile hatching rates of eggs were observed to decrease greatly in the kanamycin or tetracycline-treated group but maintained in the ampicillin or ciprofloxacin-treated group. Furthermore, the reduced hatching rates were found to be associated with density of Coxiella-LE in eggs.
conclusionsThe findings indicate that Coxiella-LE is essential for the reproduction of R. haemaphysaloides ticks, and that kanamycin can be used to study the role of Coxiella-LE on ticks.
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