ArticleBMC genomics2018
Transcriptome-wide identification of optimal reference genes for expression analysis of Pyropia yezoensis responses to abiotic stress.
Article in BMC genomics, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed.
- Article
- Physiological and molecular responses of Pyropia yezoensis thallus to high-light stress.BMC plant biology · 2025Article
- Transcriptome profiling reveals the impact of various levels of biochar application on the growth of flue-cured tobacco plants.BMC plant biology · 2024Article
- Genome-wide analysis and expression profile of the bZIP gene family inFrontiers in plant science · 2024Article
- Genome-Wide Identification and Analysis ofPlants (Basel, Switzerland) · 2023Article
- Article
- Identification and validation of the reference genes in the echiuran worm Urechis unicinctus based on transcriptome data.BMC genomics · 2023Article
- Transcriptome-Based Evaluation of Optimal Reference Genes for Quantitative Real-Time PCR in Yak Stomach throughout the Growth Cycle.Animals : an open access journal from MDPI · 2023Article
- Article
- Comparative analysis of novel and common reference genes in adult tissues of the mussel Mytilus galloprovincialis.BMC genomics · 2022Article
- Selection and validation of reference genes for quantitative real-time PCR of Quercus mongolica Fisch. ex Ledeb under abiotic stresses.PloS one · 2022Article
- Public transcriptome database-based selection and validation of reliable reference genes for breast cancer research.Biomedical engineering online · 2021Article
- Selection of a reference gene for studies on lipid-related aquatic adaptations of toothed whales (Ecology and evolution · 2021Article
- Genome-wide analysis of HSP70 gene superfamily in Pyropia yezoensis (Bangiales, Rhodophyta): identification, characterization and expression profiles in response to dehydration stress.BMC plant biology · 2021Article
- Comparative Transcriptome Profiling ofPlants (Basel, Switzerland) · 2021Article
- The Absence of Hydrodynamic Stress Promotes Acquisition of Freezing Tolerance and Freeze-Dependent Asexual Reproduction in the Red Alga 'Plants (Basel, Switzerland) · 2021Article
- Transcriptome-Based Selection and Validation of Reference Genes for Gene Expression Analysis ofFrontiers in microbiology · 2021Article
- Selection and validation of reference genes for measuring gene expression in Toona ciliata under different experimental conditions by quantitative real-time PCR analysis.BMC plant biology · 2020Article
- Transcriptome-based selection and validation of optimal house-keeping genes for skin research in goats (Capra hircus).BMC genomics · 2020Article
- Identification and evaluation of reference genes for quantitative real-time PCR analysis in Passiflora edulis under stem rot condition.Molecular biology reports · 2020Article
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5 authors.
Funding
Abstract
backgroundPyropia yezoensis, a marine red alga, is an ideal research model for studying the mechanisms of abiotic stress tolerance in intertidal seaweed. Real-time quantitative polymerase chain reaction (RT-qPCR) is the most commonly used method to analyze gene expression levels. To accurately quantify gene expression, selection and validation of stable reference genes is required.
resultsWe used transcriptome profiling data from different abiotic stress treatments to identify six genes with relatively stable expression levels: MAP, ATPase, CGS1, PPK, DPE2, and FHP. These six genes and three conventional reference genes, UBC, EF1-α, and eif4A, were chosen as candidates for optimal reference gene selection. Five common statistical approaches (geNorm, ΔCt method, NormFinder, BestKeeper, and ReFinder) were used to identify the stability of each reference gene. Our results show that: MAP, UBC, and FHP are stably expressed in all analyzed conditions; CGS1 and UBC are stably expressed under conditions of dehydration stress; and MAP, UBC, and CGS1 are stably expressed under conditions of temperature stress.
conclusionWe have identified appropriate reference genes for RT-qPCR in P. yezoensis under different abiotic stress conditions which will facilitate studies of gene expression under these conditions.
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