ArticlePloS one2018
Intestinal manipulation affects mucosal antimicrobial defense in a mouse model of postoperative ileus.
Article in PloS one, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 18 citations in OpenAlex.
- Impact of portal and systemic venous drainage on early inflammatory responses in a swine model of living donor intestinal transplantation.Clinics (Sao Paulo, Brazil) · 2026Article
- STING controls glycolysis and histone lactylation to drive macrophage metabolic reprogramming in postoperative ileus.Communications biology · 2026Article
- Machine learning prediction of sepsis in paralytic ileus using interpretable clinical models.Frontiers in cellular and infection microbiology · 2026Article
- Article
- Effect of the gut microbiota and their metabolites on postoperative intestinal motility and its underlying mechanisms.Journal of translational medicine · 2023Review
- Emerging Paradigms in the Prevention of Surgical Site Infection: The Patient Microbiome and Antimicrobial Resistance.Anesthesiology · 2022Article
- Hangekobokuto, a traditional Japanese herbal medicine, ameliorates postoperative ileus through its anti-inflammatory action.Journal of smooth muscle research = Nihon Heikatsukin Gakkai kikanshi · 2022Article
- Increased mortality and altered local immune response in secondary peritonitis after previous visceral operations in mice.Scientific reports · 2021Article
- Positioning strictureplasty in the treatment of extensive Crohn's disease ileitis: a comparative study with ileocecal resection.International journal of colorectal disease · 2021Observational
- Quality Control in Diagnostic Fluorescence In Situ Hybridization (FISH) in Microbiology.Methods in molecular biology (Clifton, N.J.) · 2021Article
- A Population of Radio-Resistant Macrophages in the Deep Myenteric Plexus Contributes to Postoperative IleusFrontiers in immunology · 2020Article
- Postoperative Ileus and Postoperative Gastrointestinal Tract Dysfunction: Pathogenic Mechanisms and Novel Treatment Strategies Beyond Colorectal Enhanced Recovery After Surgery Protocols.Frontiers in pharmacology · 2020Review
- AIM2 inflammasome-derived IL-1β induces postoperative ileus in mice.Scientific reports · 2019Article
Corrections and comments
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Authors and funding
11 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
aimTo explore the effects of abdominal surgery and interleukin-1 signaling on antimicrobial defense in a model of postoperative ileus.
methodsC57BL/6 and Interleukin-1 receptor type I (IL-1R1) deficient mice underwent intestinal manipulation to induce POI. Expression of mucosal IL-1α, IL-1β and IL-1R1 and several antimicrobial peptides and enzymes were measured by quantitative PCR or ELISA, western blotting or immunohistochemistry. Bacterial overgrowth was determined by fluorescent in-situ hybridization and counting of jejunal luminal bacteria. Translocation of aerobic and anaerobic bacteria into the intestinal wall, mesenteric lymph nodes, liver and spleen was determined by counting bacterial colonies on agar plates 48h after plating of tissue homogenates. Antimicrobial activity against E. coli and B. vulgatus was analyzed in total and cationic fractions of small bowel mucosal tissue homogenates by a flow cytometry-based bacterial depolarization assay.
resultsJejunal bacterial overgrowth was detected 24h after surgery. At the same time point, but not in the early phase 3h after surgery, bacterial translocation into the liver and mesenteric lymph nodes was observed. Increased antimicrobial activity against E. coli was induced within early phase of POI. Basal antimicrobial peptide and enzyme gene expression was higher in the ileal compared to the jejunal mucosa. The expression of lysozyme 1, cryptdin 1, cryptdin 4 and mucin 2 were reduced 24h after surgery in the ileal mucosa and mucin 2 was also reduced in the jejunum. Postoperative IL-1α and IL-1β were increased in the postoperative mucosa. Deficiency of IL-1R1 affected the expression of antimicrobial peptides during homeostasis and POI.
conclusionSmall bowel antimicrobial capacity is disturbed during POI which is accompanied by bacterial overgrowth and translocation. IL-1R1 is partially involved in the gene expression of mucosal antimicrobial peptides. Altered small bowel antimicrobial activity may contribute also to POI development and manifestation in patients undergoing abdominal surgery.
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