ArticleeLife2018
Microtubule assembly governed by tubulin allosteric gain in flexibility and lattice induced fit.
Article in eLife, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed.
- Microtubules in Martini: Parameterizing a heterogeneous elastic-network towards a mechanically accurate microtubule.PNAS nexus · 2025Article
- Microtubule dynamics are defined by conformations and stability of clustered protofilaments.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Exploring tubulin-paclitaxel binding modes through extensive molecular dynamics simulations.Scientific reports · 2025Article
- Stable GDP-tubulin islands rescue dynamic microtubules.The Journal of cell biology · 2024Article
- Structural insights into the mechanism of GTP initiation of microtubule assembly.Nature communications · 2023Article
- Effect of Colchicine Binding Site Inhibitors on the Tubulin Intersubunit Interaction.ACS omega · 2023Article
- Diverse cytomotive actins and tubulins share a polymerization switch mechanism conferring robust dynamics.Science advances · 2023Article
- Review
- Wordom update 2: A user-friendly program for the analysis of molecular structures and conformational ensembles.Computational and structural biotechnology journal · 2023Article
- Mechanism of Tubulin Oligomers and Single-Ring Disassembly Catastrophe.The journal of physical chemistry letters · 2022Article
- Bending-torsional elasticity and energetics of the plus-end microtubule tip.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Regulation of microtubule dynamics, mechanics and function through the growing tip.Nature reviews. Molecular cell biology · 2021Review
- XMAP215 promotes microtubule catastrophe by disrupting the growing microtubule end.The Journal of cell biology · 2021Article
- Atomistic molecular dynamics simulations of tubulin heterodimers explain the motion of a microtubule.European biophysics journal : EBJ · 2021Article
- Atomistic Basis of Microtubule Dynamic Instability Assessed Via Multiscale Modeling.Annals of biomedical engineering · 2021Article
- Allosteric Pathways Originating at Cysteine Residues in Regulators of G-Protein Signaling Proteins.Biophysical journal · 2021Article
- Microtubule instability driven by longitudinal and lateral strain propagation.PLoS computational biology · 2020Article
- Microtubule Simulations Provide Insight into the Molecular Mechanism Underlying Dynamic Instability.Biophysical journal · 2020Article
- Insights into allosteric control of microtubule dynamics from a buried β-tubulin mutation that causes faster growth and slower shrinkage.Protein science : a publication of the Protein Society · 2020Article
- MAP6 is an intraluminal protein that induces neuronal microtubules to coil.Science advances · 2020Article
Corrections and comments
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Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Microtubules (MTs) are key components of the cytoskeleton and play a central role in cell division and development. MT assembly is known to be associated with a structural change in [Formula: see text]-tubulin dimers from kinked to straight conformations. How GTP binding renders individual dimers polymerization-competent, however, is still unclear. Here, we have characterized the conformational dynamics and energetics of unassembled tubulin using atomistic molecular dynamics and free energy calculations. Contrary to existing allosteric and lattice models, we find that GTP-tubulin favors a broad range of almost isoenergetic curvatures, whereas GDP-tubulin has a much lower bending flexibility. Moreover, irrespective of the bound nucleotide and curvature, two conformational states exist differing in location of the anchor point connecting the monomers that affects tubulin bending, with one state being strongly favored in solution. Our findings suggest a new combined model in which MTs incorporate and stabilize flexible GTP-dimers with a specific anchor point state.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.