Evidence map›Paper›PMID 29652063›Full record

ArticleChemical communications (Cambridge, England)2018

Genetic encoding of 2-aryl-5-carboxytetrazole-based protein photo-cross-linkers.

Yulin Tian, Qing Lin

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Chemical communications (Cambridge, England), 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed.

  1. Article
  2. Article
  3. Review
  4. Review
  5. Article
  6. Lights on 2,5-diaryl tetrazoles: applications and limits of a versatile photoclick reaction.Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology · 2022
    Review
  7. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors.

Yulin TianDepartment of Chemistry, State University of New York at Buffalo, Buffalo, New York 14260-3000, USA. qinglin@buffalo.edu.
Qing Lin

Funding

Development and Applications of Photoinducible Bioorthogonal ChemistryR01GM085092 · NIGMS · STATE UNIVERSITY OF NEW YORK AT BUFFALO · PI LIN, QING · 2009 to 2017
$2.8M
NIGMS NIH HHS R01 GM085092
6 · The paper itself

Abstract

Three γ-heteroatom-substituted N-methylpyrroletetrazole-lysines (mPyTXKs) were synthesized and subsequently incorporated into proteins site-specifically via genetic code expansion. The γ-seleno-substituted derivative, mPyTSeK, showed excellent incorporation efficiency in Escherichia coli and allowed site-selective photo-cross-linking of the GST dimer. Furthermore, the mPyTSeK-cross-linked GST dimer can be cleaved under mild oxidative conditions. The incorporation of mPyTXKs into proteins in mammalian cells was also demonstrated. Lastly, the recombinantly expressed mPyTSeK-encoded Grb2 was shown to covalently capture its interaction partner, EGFR, in mammalian cell lysate, which was subsequently released after treatment with H2O2.

Indexed as

Protein EngineeringAnimalsCross-Linking ReagentsErbB ReceptorsEscherichia coliGlutathione TransferaseGRB2 Adaptor ProteinHEK293 CellsHumansHydrogen PeroxideLysineSchistosoma japonicumTetrazolesUltraviolet RaysCross-Linking ReagentsEGFR protein, humanErbB ReceptorsGlutathione TransferaseGRB2 Adaptor ProteinGRB2 protein, humanHydrogen PeroxideLysineTetrazoles

Identifiers

PMID29652063
PMCPMC5921928

What OpenQuestion holds

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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.