ArticleEuropean journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology2018
Significant increase in cultivation of Gardnerella vaginalis, Alloscardovia omnicolens, Actinotignum schaalii, and Actinomyces spp. in urine samples with total laboratory automation.
Article in European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed.
- Occurrence and relevance of Gardnerella vaginalis in urine cultures of kidney transplant recipients- a retrospective cohort analysis.BMC microbiology · 2026Article
- Total Laboratory Automation for Microbiology Activities: An HTA Analysis in an Italian Laboratory Setting.ClinicoEconomics and outcomes research : CEOR · 2026Article
- Multi-omics profiling reveals distinct pathogenic mechanisms in Hunner and non-Hunner interstitial cystitis subtypes.Scientific reports · 2025Article
- Microbiota Ecosystem in Recurrent Cystitis and the Immunological Microenvironment of Urothelium.Healthcare (Basel, Switzerland) · 2023Review
- Stop Waiting for Tomorrow: Disk Diffusion Performed on Early Growth Is an Accurate Method for Antimicrobial Susceptibility Testing with Reduced Turnaround Time.Journal of clinical microbiology · 2022Article
- Total Laboratory Automation for Rapid Detection and Identification of Microorganisms and Their Antimicrobial Resistance Profiles.Frontiers in cellular and infection microbiology · 2022Review
- Genomic Investigation and Successful Containment of an Intermittent Common Source Outbreak of OXA-48-Producing Enterobacter cloacae Related to Hospital Shower Drains.Microbiology spectrum · 2021Article
- Culturomics Approaches Expand the Diagnostic Accuracy for Sexually Transmitted Infections.International journal of molecular sciences · 2021Review
- Article
- Laboratory Automation in the Microbiology Laboratory: an Ongoing Journey, Not a Tale?Journal of clinical microbiology · 2021Article
- Total Laboratory Automation and Three Shifts Reduce Turnaround Time of Cerebrospinal Fluid Culture Results in the Chinese Clinical Microbiology Laboratory.Frontiers in cellular and infection microbiology · 2021Article
- The Role of Gut, Vaginal, and Urinary Microbiome in Urinary Tract Infections: From Bench to Bedside.Diagnostics (Basel, Switzerland) · 2020Review
- Recurrent Escherichia coli Urinary Tract Infection Triggered by Gardnerella vaginalis Bladder Exposure in Mice.Journal of visualized experiments : JoVE · 2020Article
- Consolidation of Clinical Microbiology Laboratories and Introduction of Transformative Technologies.Clinical microbiology reviews · 2020Review
- Reincubation of culture-negative urines for an additional 20 hours does not identify additional UTI cases.Journal of medical microbiology · 2020Article
- Evaluation of WASPLab Software To Automatically Read chromID CPS Elite Agar for Reporting of Urine Cultures.Journal of clinical microbiology · 2019Article
- Reducing the time between inoculation and first-read of urine cultures using total lab automation significantly reduces turn-around-time of positive culture results with minimal loss of first-read sensitivity.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2019Article
- Impact of total laboratory automation on workflow and specimen processing time for culture of urine specimens.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2018Article
- Laboratory Automation in Clinical Microbiology.Bioengineering (Basel, Switzerland) · 2018Review
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Authors and funding
6 authors.
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Abstract
While total laboratory automation (TLA) is well established in laboratory medicine, only a few microbiological laboratories are using TLA systems. Especially in terms of speed and accuracy, working with TLA is expected to be superior to conventional microbiology. We compared in total 35,564 microbiological urine cultures with and without incubation and processing with BD Kiestra TLA for a 6-month period each retrospectively. Sixteen thousand three hundred thirty-eight urine samples were analyzed in the pre-TLA period and 19,226 with TLA. Sixty-two percent (n = 10,101/16338) of the cultures processed without TLA and 68% (n = 13,102/19226) of the cultures processed with TLA showed growth. There were significantly more samples with two or more species per sample and with low numbers of colony forming units (CFU) after incubation with TLA. Regarding the type of bacteria, there were comparable amounts of Enterobacteriaceae in the samples, slightly less non-fermenting Gram-negative bacteria, but significantly more Gram-positive cocci, and Gram-positive rods. Especially Alloscardivia omnicolens, Gardnerella vaginalis, Actinomyces spp., and Actinotignum schaalii were significantly more abundant in the samples incubated and processed with TLA. The time to report was significantly lower in the TLA processed samples by 1.5 h. We provide the first report in Europe of a large number of urine samples processed with TLA. TLA showed enhanced growth of non-classical and rarely cultured bacteria from urine samples. Our findings suggest that previously underestimated bacteria may be relevant pathogens for urinary tract infections. Further studies are needed to confirm our findings.
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