ArticleFrontiers in cellular neuroscience2018
Selective Labeling of Individual Neurons in Dense Cultured Networks With Nanoparticle-Enhanced Photoporation.
Article in Frontiers in cellular neuroscience, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 27 citations in OpenAlex.
- Photodisruption of the Inner Limiting Membrane Promotes Retinal Engraftment of Stem-Cell Derived Retinal Ganglion Cells.Investigative ophthalmology & visual science · 2026Article
- Cas9 RNP transfection by vapor nanobubble photoporation forMolecular therapy. Nucleic acids · 2021Article
- FAM19A5/TAFA5, a novel neurokine, plays a crucial role in depressive-like and spatial memory-related behaviors in mice.Molecular psychiatry · 2021Article
- Intracellular Delivery of mRNA in Adherent and Suspension Cells by Vapor Nanobubble Photoporation.Nano-micro letters · 2020Article
- Long-term live-cell microscopy with labeled nanobodies delivered by laser-induced photoporation.Nano research · 2020Article
- Nanotheranostic Applications for Detection and Targeting Neurodegenerative Diseases.Frontiers in neuroscience · 2020Review
- Laser-induced vapor nanobubbles improve diffusion in biofilms of antimicrobial agents for wound care.Biofilm · 2019Article
- Delivery of Mixed-Lineage Kinase Domain-Like Protein by Vapor Nanobubble Photoporation Induces Necroptotic-Like Cell Death in Tumor Cells.International journal of molecular sciences · 2019Article
- Hierarchy of Hybrid Materials-The Place of Inorganics-Frontiers in chemistry · 2019Review
- Polydopamine-coated Au nanorods for targeted fluorescent cell imaging and photothermal therapy.Beilstein journal of nanotechnology · 2019Article
- Image-Based Profiling of Synaptic Connectivity in Primary Neuronal Cell Culture.Frontiers in neuroscience · 2018Review
- Repeated photoporation with graphene quantum dots enables homogeneous labeling of live cells with extrinsic markers for fluorescence microscopy.Light, science & applications · 2018Article
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 3 countries.
Funding
Abstract
Neurodevelopmental and neurodegenerative disorders are characterized by subtle alterations in synaptic connections and perturbed neuronal network functionality. A hallmark of neuronal connectivity is the presence of dendritic spines, micron-sized protrusions of the dendritic shaft that compartmentalize single synapses to fine-tune synaptic strength. However, accurate quantification of spine density and morphology in mature neuronal networks is hampered by the lack of targeted labeling strategies. To resolve this, we have optimized a method to deliver cell-impermeable compounds into selected cells based on Spatially resolved NAnoparticle-enhanced Photoporation (SNAP). We show that SNAP enables efficient labeling of selected individual neurons and their spines in dense cultured networks without affecting short-term viability. We compare SNAP with widely used spine labeling techniques such as the application of lipophilic dyes and genetically encoded fluorescent markers. Using SNAP, we demonstrate a time-dependent increase in spine density in healthy cultures as well as a reduction in spine density after chemical mimicry of hypoxia. Since the sparse labeling procedure can be automated using an intelligent acquisition scheme, SNAP holds promise for high-content screening campaigns of neuronal connectivity in the context of neurodevelopmental and neurodegenerative disorders.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.