Evidence map›Paper›PMID 29651133›Full record

ArticleScientific reports2018

Refined protocols of tamoxifen injection for inducible DNA recombination in mouse astroglia.

Hannah M Jahn, Carmen V Kasakow, Andreas Helfer, Julian Michely, Alexei Verkhratsky, Hans H Maurer, Anja Scheller, Frank Kirchhoff

Open access · goldAbstract read
In one paragraph

Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 93 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
93citing papers in PubMed, 1 pooled it
5.2field-weighted citation impact, top 4% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

93 citing papers in PubMed, 1 synthesis or guideline pooled it, 135 citations in OpenAlex.

  1. CONature communications · 2022
    Pooled it
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  8. CD38 endows local antigen-specific TNature immunology · 2026
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  17. Autophagy Regulates Müller Glial Cell Inflammatory Activation.Investigative ophthalmology & visual science · 2025
    Article
  18. Neurophotonics · 2025
    Article
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33 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 2 institutions in 2 countries.

Hannah M JahnMolecular Physiology, Center for Integrative Physiology and Molecular Medicine (CIPMM), University of Saarland, 66421 Homburg, Germany.
Carmen V KasakowMolecular Physiology, Center for Integrative Physiology and Molecular Medicine (CIPMM), University of Saarland, 66421 Homburg, Germany.
Andreas HelferDepartment of Experimental and Clinical Toxicology, University of Saarland, 66421, Homburg, Germany.
Julian MichelyDepartment of Experimental and Clinical Toxicology, University of Saarland, 66421, Homburg, Germany.
Alexei VerkhratskyFaculty of Biology, Medicine and Health, The University of Manchester, Manchester M13 9PL, UK.
Hans H MaurerDepartment of Experimental and Clinical Toxicology, University of Saarland, 66421, Homburg, Germany.
Anja SchellerMolecular Physiology, Center for Integrative Physiology and Molecular Medicine (CIPMM), University of Saarland, 66421 Homburg, Germany.ORCID 0000-0001-8955-2634
Frank KirchhoffMolecular Physiology, Center for Integrative Physiology and Molecular Medicine (CIPMM), University of Saarland, 66421 Homburg, Germany. frank.kirchhoff@uks.eu.ORCID 0000-0002-2324-2761
Saarland University · DEUniversity of Manchester · GB

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Inducible DNA recombination of floxed alleles in vivo by liver metabolites of tamoxifen (TAM) is an important tool to study gene functions. Here, we describe protocols for optimal DNA recombination in astrocytes, based on the GLAST-Cre

Indexed as

AllelesAnimalsAstrocytesBrainCerebellumChromatography, LiquidDNALiverMiceReceptors, AMPAReceptors, GABA-BReceptors, Purinergic P2Y1Recombination, GeneticRNA, UntranslatedTamoxifenTandem Mass Spectrometry4-hydroxy-N-desmethyltamoxifenafimoxifeneDNAGt(ROSA)26Sor non-coding RNA, mouseP2ry1 protein, mouseReceptors, AMPAReceptors, GABA-BReceptors, Purinergic P2Y1RNA, UntranslatedTamoxifen

Identifiers

PMID29651133
PMCPMC5897555
OpenAlexW2797019545

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.