ArticleThe EMBO journal2018
Real-time observation of flexible domain movements in CRISPR-Cas9.
Article in The EMBO journal, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed.
- Structural basis for inhibition of SpyCas9 by the anti-CRISPR protein AcrIIA26.The Biochemical journal · 2026Article
- And… cut! - how conformational regulation of CRISPR-Cas effectors directs nuclease activity.The Biochemical journal · 2025Review
- Structural basis of Cas9 DNA interrogation with a 5' truncated sgRNA.Nucleic acids research · 2025Article
- Probing Electrostatic Interactions in DNA-Bound CRISPR/Cas9 Complexes by Molecular Dynamics Simulations.ACS omega · 2024Article
- Insights into the Mechanism of CRISPR/Cas9-Based Genome Editing from Molecular Dynamics Simulations.ACS omega · 2023Review
- Probing the Dynamics ofInternational journal of molecular sciences · 2022Article
- Molecular mechanisms ofBiophysics reports · 2021Article
- Heterogeneous dissociation process of truncated RNAs by oligomerized Vasa helicase.Communications biology · 2021Article
- Efficient DNA interrogation of SpCas9 governed by its electrostatic interaction with DNA beyond the PAM and protospacer.Nucleic acids research · 2021Article
- Probing the stability of the SpCas9-DNA complex after cleavage.Nucleic acids research · 2021Article
- Engineering of the genome editing protein Cas9 to slide along DNA.Scientific reports · 2021Article
- Real-time observation of Cas9 postcatalytic domain motions.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- DeepFRET, a software for rapid and automated single-molecule FRET data classification using deep learning.eLife · 2020Article
- Allosteric regulation of CRISPR-Cas9 for DNA-targeting and cleavage.Current opinion in structural biology · 2020Review
- Allosteric Motions of the CRISPR-Cas9 HNH Nuclease Probed by NMR and Molecular Dynamics.Journal of the American Chemical Society · 2020Article
- Coevolutionary Couplings Unravel PAM-Proximal Constraints of CRISPR-SpCas9.Biophysical journal · 2019Article
- The post-PAM interaction of RNA-guided spCas9 with DNA dictates its target binding and dissociation.Science advances · 2019Article
- Article
- Structural and functional insights into theeLife · 2019Article
- Dynamics of Cas10 Govern Discrimination between Self and Non-self in Type III CRISPR-Cas Immunity.Molecular cell · 2019Article
Corrections and comments
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The CRISPR-associated protein Cas9 is widely used for genome editing because it cleaves target DNA through the assistance of a single-guide RNA (sgRNA). Structural studies have revealed the multi-domain architecture of Cas9 and suggested sequential domain movements of Cas9 upon binding to the sgRNA and the target DNA These studies also hinted at the flexibility between domains; however, it remains unclear whether these flexible movements occur in solution. Here, we directly observed dynamic fluctuations of multiple Cas9 domains, using single-molecule FRET We found that the flexible domain movements allow Cas9 to adopt transient conformations beyond those captured in the crystal structures. Importantly, the HNH nuclease domain only accessed the DNA cleavage position during such flexible movements, suggesting the importance of this flexibility in the DNA cleavage process. Our FRET data also revealed the conformational flexibility of apo-Cas9, which may play a role in the assembly with the sgRNA Collectively, our results highlight the potential role of domain fluctuations in driving Cas9-catalyzed DNA cleavage.
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