ArticleBiophysical journal2018
SPT and Imaging FCS Provide Complementary Information on the Dynamics of Plasma Membrane Molecules.
Article in Biophysical journal, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- Super-resolution algorithms for imaging FCS enhancement: A comparative study.Biophysical journal · 2025Article
- Disentangling Timescales of Molecular Kinetics with spFRET using ALEX-FCS.Journal of fluorescence · 2025Article
- Crossover patterning through condensation and coarsening of pro-crossover factors.Nature cell biology · 2025Article
- Dissecting the biophysical mechanisms of oleate hydratase association with membranes.Frontiers in molecular biosciences · 2024Article
- Combined SPT and FCS methods reveal a mechanism of RNAP II oversampling in cell nuclei.Scientific reports · 2023Article
- Current capabilities and future perspectives of FCS: super-resolution microscopy, machine learning, and in vivo applications.Communications biology · 2023Review
- Biased activation of the receptor tyrosine kinase HER2.Cellular and molecular life sciences : CMLS · 2023Article
- DynamicNano letters · 2022Article
- Unraveling the hidden temporal range of fast βCommunications biology · 2022Article
- Simultaneous spatiotemporal super-resolution and multi-parametric fluorescence microscopy.Nature communications · 2021Article
- Membrane dynamics are slowed for Alexa594-labeled membrane proteins due to substrate interactions.BBA advances · 2021Article
- Fluorescence strategies for mapping cell membrane dynamics and structures.APL bioengineering · 2020Article
- Article
- Synthetic and genetic dimers as quantification ruler for single-molecule counting with PALM.Molecular biology of the cell · 2019Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The dynamics of biomolecules in the plasma membrane is of fundamental importance to understanding cellular processes. Cellular signaling often starts with extracellular ligand binding to a membrane receptor, which then transduces an intracellular signal. Ligand binding and receptor-complex activation often involve a complex rearrangement of proteins in the membrane, which results in changes in diffusion properties. Two widely used methods to characterize biomolecular diffusion are single-particle tracking (SPT) and imaging total internal reflection fluorescence correlation spectroscopy (ITIR-FCS). Here, we compare the results of recovered diffusion coefficients and mean-square displacements of the two methods by simulations of free, domain-confined, or meshwork diffusion. We introduce, to our knowledge, a new method for the determination of confinement radii from ITIR-FCS data. We further establish and demonstrate simultaneous SPT/ITIR-FCS for direct comparison within living cells. Finally, we compare the results obtained by SPT and ITIR-FCS for the receptor tyrosine kinase MET. Our results show that SPT and ITIR-FCS yield complementary information on diffusion properties of biomolecules in cell membranes.
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