ArticleBiophysical journal2018
Protein Corona in Response to Flow: Effect on Protein Concentration and Structure.
Article in Biophysical journal, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
20 citing papers in PubMed.
- Tuning protein corona on nucleic acid nanodrugs for targeted delivery.Acta pharmaceutica Sinica. B · 2026Review
- Using a bacterial protein to selectively target bacterial biofilms: Treatment of S. epidermidis biofilms with targeted Photothermal gold nanoparticles.Journal of colloid and interface science · 2026Article
- Electrostatic adsorption of polyanions onto lipid nanoparticles controls uptake, trafficking, and transfection of RNA and DNA therapies.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Interaction of Graphene Oxide Particles and Dendrimers with Human Breast Cancer Cells by Real-Time Microscopy.Pharmaceutics · 2023Article
- Mesenchymal Stem Cell Culture within Perfusion Bioreactors Incorporating 3D-Printed Scaffolds Enables Improved Extracellular Vesicle Yield with Preserved Bioactivity.Advanced healthcare materials · 2023Article
- Assessment of anti-inflammatory bioactivity of extracellular vesicles is susceptible to error via media component contamination.Cytotherapy · 2023Article
- Nanoparticle protein corona: from structure and function to therapeutic targeting.Lab on a chip · 2023Review
- Concentration and composition of the protein corona as a function of incubation time and serum concentration: an automated approach to the protein corona.Analytical and bioanalytical chemistry · 2022Article
- Vascular bifurcation influences the protein corona composition on nanoparticles and impacts their cellular uptake.Nanoscale advances · 2022Article
- A novel insight into mechanism of derangement of coagulation balance: interactions of quantum dots with coagulation-related proteins.Particle and fibre toxicology · 2022Article
- Performance of nanoparticles for biomedical applications: TheBiophysics reviews · 2022Review
- Environmental dimensions of the protein corona.Nature nanotechnology · 2021Review
- Biocompatibility of nanomaterials and their immunological properties.Biomedical materials (Bristol, England) · 2021Review
- DNA-nanoparticle interactions: Formation of a DNA corona and its effects on a protein corona.Biointerphases · 2020Article
- Automation and low-cost proteomics for characterization of the protein corona: experimental methods for big data.Analytical and bioanalytical chemistry · 2020Article
- Unveiling the pitfalls of the protein corona of polymeric drug nanocarriers.Drug delivery and translational research · 2020Review
- Polarimetric Measurements of Surface Chirality Based on Linear and Nonlinear Light Scattering.Frontiers in chemistry · 2020Review
- Engineered Polymeric Materials for Biological Applications: Overcoming Challenges of the Bio-Nano Interface.Polymers · 2019Review
- Shear-Induced Extensional Response Behaviors of Tethered von Willebrand Factor.Biophysical journal · 2019Article
- Target Site Delivery and Residence of Nanomedicines: Application of Quantitative Systems Pharmacology.Pharmacological reviews · 2019Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Nanoparticles used in cellular applications encounter free serum proteins that adsorb onto the surface of the nanoparticle, forming a protein corona. This protein layer controls the interaction of nanoparticles with cells. For nanomedicine applications, it is important to consider how intravenous injection and the subsequent shear flow will affect the protein corona. Our goal was to determine if shear flow changed the composition of the protein corona and if these changes affected cellular binding. Colorimetric assays of protein concentration and gel electrophoresis demonstrate that polystyrene nanoparticles subjected to flow have a greater concentration of serum proteins adsorbed on the surface, especially plasminogen. Plasminogen, in the absence of nanoparticles, undergoes changes in structure in response to flow, characterized by fluorescence and circular dichroism spectroscopy. The protein-nanoparticle complexes formed from fetal bovine serum after flow had decreased cellular binding, as measured with flow cytometry. In addition to the relevance for nanomedicine, these results also highlight the technical challenges of protein corona studies. The composition of the protein corona was highly dependent on the initial mixing step: rocking, vortexing, or flow. Overall, these results reaffirm the importance of the protein corona in nanoparticle-cell interactions and point toward the challenges of predicting corona composition based on nanoparticle properties.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.