ArticlePloS one2018
A low dose lipid infusion is sufficient to induce insulin resistance and a pro-inflammatory response in human subjects.
Article in PloS one, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
What it found
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Who cites it
15 citing papers in PubMed, 28 citations in OpenAlex.
- Trial
- A randomised trial examining inflammatory signaling in acutely induced hyperinsulinemia and hyperlipidemia in normal weight women-the reprometabolic syndrome.PloS one · 2021 · on this mapTrial
- 16S rRNA Gene Amplicon Sequencing of Gut Microbiota in Gestational Diabetes Mellitus and Type 2 Diabetes Mellitus.Journal of diabetes research · 2026Article
- Ketogenic propensity is differentially related to lipid-induced hepatic and peripheral insulin resistance.Acta physiologica (Oxford, England) · 2023Article
- The Impacts of Animal-Based Diets in Cardiovascular Disease Development: A Cellular and Physiological Overview.Journal of cardiovascular development and disease · 2023Review
- TLR13 contributes to skeletal muscle atrophy by increasing insulin resistance in chronic kidney disease.Cell proliferation · 2022Article
- Review
- The Novel Role of Metabolism-Associated Molecular Patterns in Sepsis.Frontiers in cellular and infection microbiology · 2022Review
- Dietary Component-Induced Inflammation and Its Amelioration by Prebiotics, Probiotics, and Synbiotics.Frontiers in nutrition · 2022Review
- Putting ATM to BED: How Adipose Tissue Macrophages Are Affected by Bariatric Surgery, Exercise, and Dietary Fatty Acids.Advances in nutrition (Bethesda, Md.) · 2021Review
- Berberine improves intralipid-induced insulin resistance in murine.Acta pharmacologica Sinica · 2021Article
- The Potential Role of RP105 in Regulation of Inflammation and Osteoclastogenesis During Inflammatory Diseases.Frontiers in cell and developmental biology · 2021Review
- The intestinal microbiota fuelling metabolic inflammation.Nature reviews. Immunology · 2020Review
- Interactive association of lipopolysaccharide and free fatty acid with the prevalence of type 2 diabetes: A community-based cross-sectional study.Journal of diabetes investigation · 2019Article
- Elevated free fatty acid level is associated with insulin-resistant state in nondiabetic Chinese people.Diabetes, metabolic syndrome and obesity : targets and therapy · 2019Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
Abstract
objectiveThe root cause behind the low-grade inflammatory state seen in insulin resistant (obesity and type 2 diabetes) states is unclear. Insulin resistant subjects have elevations in plasma free fatty acids (FFA), which are ligands for the pro-inflammatory toll-like receptor (TLR)4 pathway. We tested the hypothesis that an experimental elevation in plasma FFA (within physiological levels) in lean individuals would upregulate TLR4 and activate downstream pathways (e.g., MAPK) in circulating monocytes. RESEARCH DESIGN AND
methodsTwelve lean, normal glucose-tolerant subjects received a low dose (30 ml/h) 48 h lipid or saline infusion on two different occasions. Monocyte TLR4 protein level, MAPK phosphorylation, and expression of genes in the TLR pathway were determined before and after each infusion.
resultsThe lipid infusion significantly increased monocyte TLR4 protein and phosphorylation of JNK and p38 MAPK. Lipid-mediated increases in TLR4 and p38 phosphorylation directly correlated with reduced peripheral insulin sensitivity (M value). Lipid increased levels of multiple genes linked to inflammation, including several TLRs, CD180, MAP3K7, and CXCL10. Monocytes exposed in vivo to lipid infusion exhibited enhanced in vitro basal and LPS-stimulated IL-1β secretion.
conclusionsIn lean subjects, a small increase in plasma FFA (as seen in insulin resistant subjects) is sufficient to upregulate TLR4 and stimulate inflammatory pathways (MAPK) in monocytes. Moreover, lipids prime monocytes to endotoxin. We provide proof-of-concept data in humans indicating that the low-grade inflammatory state characteristic of obesity and type 2 diabetes could be caused (at least partially) by pro-inflammatory monocytes activated by excess lipids present in these individuals.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.