ArticlePLoS neglected tropical diseases2018
Gene target selection for loop-mediated isothermal amplification for rapid discrimination of Treponema pallidum subspecies.
Article in PLoS neglected tropical diseases, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed.
- External quality assessment for yaws elimination in low- and middle-income countries using plasmid-based proficiency test items.PLoS neglected tropical diseases · 2026Article
- Pathogen-Specific Electrochemical Real-Time LAMP Detection Using Universal Solid-Phase Probes on Carbon Electrodes.ACS sensors · 2025Article
- Prospective evaluation of non-invasive saliva specimens for the diagnosis of syphilis and molecular surveillance ofJournal of clinical microbiology · 2024Article
- Multiplex Loop-Mediated Isothermal Amplification Coupled Lateral Flow Assay for Point-of-Care Detection of Syphilis.Indian journal of microbiology · 2024Article
- A suite of PCR-LwCas13a assays for detection and genotyping of Treponema pallidum in clinical samples.Nature communications · 2022Article
- RLEP LAMP for the laboratory confirmation of leprosy: towards a point-of-care test.BMC infectious diseases · 2021Article
- Multiplex Mediator Displacement Loop-Mediated Isothermal Amplification for Detection of Treponema pallidum and Haemophilus ducreyi.Emerging infectious diseases · 2020Article
- A Metataxonomic Tool to Investigate the Diversity ofFrontiers in microbiology · 2019Article
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We show proof of concept for gene targets (polA, tprL, and TP_0619) that can be used in loop-mediated isothermal amplification (LAMP) assays to rapidly differentiate infection with any of the three Treponema pallidum subspecies (pallidum (TPA), pertenue (TPE), and endemicum (TEN)) and which are known to infect humans and nonhuman primates (NHPs). Four TPA, six human, and two NHP TPE strains, as well as two human TEN strains were used to establish and validate the LAMP assays. All three LAMP assays were highly specific for the target DNA. Amplification was rapid (5-15 min) and within a range of 10E+6 to 10E+2 of target DNA molecules. Performance in NHP clinical samples was similar to the one seen in human TPE strains. The newly designed LAMP assays provide proof of concept for a diagnostic tool that enhances yaws clinical diagnosis. It is highly specific for the target DNA and does not require expensive laboratory equipment. Test results can potentially be interpreted with the naked eye, which makes it suitable for the use in remote clinical settings.
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