ArticlePlant & cell physiology2018
The Basic Helix-Loop-Helix Transcription Factor GubHLH3 Positively Regulates Soyasaponin Biosynthetic Genes in Glycyrrhiza uralensis.
Article in Plant & cell physiology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 34 papers.
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34 citing papers in PubMed, 98 citations in OpenAlex.
- Complete biosynthesis of the clinical agent escin Ia.Nature communications · 2026Article
- Programmable saponin biosynthesis from gene networks to predictive biomanufacturing.Frontiers in plant science · 2026Review
- Analysis of Non-Polar Low-Molecular Metabolites in Citron (International journal of molecular sciences · 2025Article
- Glycyrrhiza, a commonly used medicinal herb: Review of species classification, pharmacology, active ingredient biosynthesis, and synthetic biology.Journal of advanced research · 2025Review
- Transcriptome‑wide excavation and expression pattern analysis of the NAC transcription factors in methyl jasmonate- and sodium chloride-induced Glycyrrhiza uralensis.Scientific reports · 2025Article
- Synthetic biology in plants.Plant biotechnology (Tokyo, Japan) · 2024Article
- RNA sequencing analysis revealsHorticulture research · 2024Article
- Glycyrrhizin Production in Licorice Hairy Roots Based on Metabolic Redirection of Triterpenoid Biosynthetic Pathway by Genome Editing.Plant & cell physiology · 2024Article
- Genome-wide identification and expression analysis of bHLH gene family revealed their potential roles in abiotic stress response, anthocyanin biosynthesis and trichome formation inFrontiers in plant science · 2024Article
- Genome-wide identification of SrbHLH transcription factors highlights its potential role in rebaudioside A (RA) biosynthesis in Stevia rebaudiana.BMC plant biology · 2023Article
- Multilayered regulation of secondary metabolism in medicinal plants.Molecular horticulture · 2023Review
- OeBAS and CYP716C67 catalyze the biosynthesis of health-beneficial triterpenoids in olive (Olea europaea) fruits.The New phytologist · 2023Article
- Methyl Jasmonate- and Salicylic Acid-Induced Transcription FactorInternational journal of molecular sciences · 2023Article
- Metabolomics reveals the response of hydroprimed maize to mitigate the impact of soil salinization.Frontiers in plant science · 2023Article
- Transcriptomics and metabolomics reveal the primary and secondary metabolism changes inFrontiers in plant science · 2023Article
- Genome-Wide Identification of SnRK1 Catalytic α Subunit and FLZ Proteins inInternational journal of molecular sciences · 2022Article
- Saponin Biosynthesis in Pulses.Plants (Basel, Switzerland) · 2022Review
- Genetic divergence in transcriptional regulators of defense metabolism: insight into plant domestication and improvement.Plant molecular biology · 2022Review
- De novo assembly and analysis of Polygonatum cyrtonema Hua and identification of genes involved in polysaccharide and saponin biosynthesis.BMC genomics · 2022Article
- Integrative analysis of microRNAs and mRNAs reveals the regulatory networks of triterpenoid saponin metabolism in Soapberry (Frontiers in plant science · 2022Article
Corrections and comments
- Erratum issuedErratum.2018
Authors and funding
11 authors at 6 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Glycyrrhiza uralensis (licorice) is a widely used medicinal plant belonging to the Fabaceae. Its main active component, glycyrrhizin, is an oleanane-type triterpenoid saponin widely used as a medicine and as a natural sweetener. Licorice also produces other triterpenoids, including soyasaponins. Recent studies have revealed various oxidosqualene cyclases and cytochrome P450 monooxygenases (P450s) required for the biosynthesis of triterpenoids in licorice. Of these enzymes, β-amyrin synthase (bAS) and β-amyrin C-24 hydroxylase (CYP93E3) are involved in the biosynthesis of soyasapogenol B (an aglycone of soyasaponins) from 2,3-oxidosqualene. Although these biosynthetic enzyme genes are known to be temporally and spatially expressed in licorice, the regulatory mechanisms underlying their expression remain unknown. Here, we identified a basic helix-loop-helix (bHLH) transcription factor, GubHLH3, that positively regulates the expression of soyasaponin biosynthetic genes. GubHLH3 preferentially activates transcription from promoters of CYP93E3 and CYP72A566, the second P450 gene newly identified and shown to be responsible for C-22β hydroxylation in soyasapogenol B biosynthesis, in transient co-transfection assays of promoter-reporter constructs and transcription factors. Overexpression of GubHLH3 in transgenic hairy roots of G. uralensis enhanced the expression levels of bAS, CYP93E3 and CYP72A566. Moreover, soyasapogenol B and sophoradiol (22β-hydroxy-β-amyrin), an intermediate between β-amyrin and soyasapogenol B, were increased in transgenic hairy root lines overexpressing GubHLH3. We found that soyasaponin biosynthetic genes and GubHLH3 were co-ordinately up-regulated by methyl jasmonate (MeJA). These results suggest that GubHLH3 regulates MeJA-responsive expression of soyasaponin biosynthetic genes in G. uralensis. The regulatory mechanisms of triterpenoid biosynthesis in legumes are compared and discussed.
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