Evidence map›Paper›PMID 29645331›Full record

ArticleChembiochem : a European journal of chemical biology2018

A Genetically Encoded Ratiometric pH Probe: Wavelength Regulation-Inspired Design of pH Indicators.

Tetyana Berbasova, Setare Tahmasebi Nick, Meisam Nosrati, Zahra Nossoni, Elizabeth M Santos, Chrysoula Vasileiou, James H Geiger, Babak Borhan

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Chembiochem : a European journal of chemical biology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed.

  1. Photoisomerization detected in a fully wavelength-tunable rhodopsin mimic system.Acta crystallographica. Section D, Structural biology · 2026
    Article
  2. Article
  3. Article
  4. Review
  5. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Tetyana BerbasovaDepartment of Chemistry, Michigan State University, East Lansing, MI, 48824, USA.
Setare Tahmasebi NickDepartment of Chemistry, Michigan State University, East Lansing, MI, 48824, USA.
Meisam NosratiDepartment of Chemistry, Michigan State University, East Lansing, MI, 48824, USA.
Zahra NossoniDepartment of Chemistry, Michigan State University, East Lansing, MI, 48824, USA.
Elizabeth M SantosDepartment of Chemistry, Michigan State University, East Lansing, MI, 48824, USA.
Chrysoula VasileiouDepartment of Chemistry, Michigan State University, East Lansing, MI, 48824, USA.
James H GeigerDepartment of Chemistry, Michigan State University, East Lansing, MI, 48824, USA.
Babak BorhanDepartment of Chemistry, Michigan State University, East Lansing, MI, 48824, USA.ORCID 0000-0002-3193-0732

Funding

Protein/Chromphore Interactions via Protein Design: Interrogation and ApplicationR01GM101353 · NIGMS · MICHIGAN STATE UNIVERSITY · PI BORHAN, BABAK, GEIGER, JAMES H · 2013 to 2022
$2.6M
NIGMS NIH HHS R01 GM101353
6 · The paper itself

Abstract

Mutants of human cellular retinol-binding protein II (hCRBPII) were engineered to bind a julolidine retinal analogue for the purpose of developing a ratiometric pH sensor. The design relied on the electrostatic influence of a titratable amino acid side chain, which affects the absorption and, thus, the emission of the protein/fluorophore complex. The ratio of emissions obtained at two excitation wavelengths that correspond to the absorption of the two forms of the protein/fluorophore complex, leads to a concentration-independent measure of pH.

Indexed as

Biosensing TechniquesFluorescenceFluorescent DyesHumansHydrogen-Ion ConcentrationModels, MolecularMutagenesis, Site-DirectedProtein ConformationRetinaldehydeRetinol-Binding Proteins, CellularSpectrometry, FluorescenceFluorescent DyesRetinaldehydeRetinol-Binding Proteins, CellularhCRBPIIjulolidinepH probeprotein engineeringratiometric sensors

Identifiers

PMID29645331
PMCPMC6742514

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.