ArticleApplied microbiology and biotechnology2018
Assessment of the scalability of a microtiter plate system for screening of oleaginous microorganisms.
Article in Applied microbiology and biotechnology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
16 citing papers in PubMed, 38 citations in OpenAlex.
- Effect of hydrogen peroxide and carbon-to-nitrogen ratio on growth and biochemical profile in oleaginous mucoromycota.Microbial cell factories · 2025Article
- Enhanced co-production of extracellular biopolymers and intracellular lipids by Rhodotorula using lignocellulose hydrolysate and fish oil by-product urea.Biotechnology for biofuels and bioproducts · 2025Article
- 'Small volume-big problem': culturing Yarrowia lipolytica in high-throughput micro-formats.Microbial cell factories · 2024Review
- Explorative characterization and taxonomy-aligned comparison of alterations in lipids and other biomolecules in Antarctic bacteria grown at different temperatures.Environmental microbiology reports · 2024Article
- Global biochemical profiling of fast-growing Antarctic bacteria isolated from meltwater ponds by high-throughput FTIR spectroscopy.PloS one · 2024Article
- Workflow for shake flask and plate cultivations with fats for polyhydroxyalkanoate bioproduction.Applied microbiology and biotechnology · 2023Article
- Lab-scale photobioreactor systems: principles, applications, and scalability.Bioprocess and biosystems engineering · 2022Review
- Advances in improving the biotechnological application of oleaginous fungus Mortierella alpina.Applied microbiology and biotechnology · 2021Review
- Assessment of Biotechnologically Important Filamentous Fungal Biomass by Fourier Transform Raman Spectroscopy.International journal of molecular sciences · 2021Article
- Calcium Affects Polyphosphate and Lipid Accumulation in Mucoromycota Fungi.Journal of fungi (Basel, Switzerland) · 2021Article
- Evaluation and optimisation of direct transesterification methods for the assessment of lipid accumulation in oleaginous filamentous fungi.Microbial cell factories · 2021Article
- Isolation and characterization of fast-growing green snow bacteria from coastal East Antarctica.MicrobiologyOpen · 2021Article
- Metal and Phosphate Ions Show Remarkable Influence on the Biomass Production and Lipid Accumulation in OleaginousJournal of fungi (Basel, Switzerland) · 2020Article
- The influence of phosphorus source and the nature of nitrogen substrate on the biomass production and lipid accumulation in oleaginous Mucoromycota fungi.Applied microbiology and biotechnology · 2020Article
- Revealing the Potential of Lipid and β-Glucans Coproduction inMicroorganisms · 2020Article
- Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
Recent developments in molecular biology and metabolic engineering have resulted in a large increase in the number of strains that need to be tested, positioning high-throughput screening of microorganisms as an important step in bioprocess development. Scalability is crucial for performing reliable screening of microorganisms. Most of the scalability studies from microplate screening systems to controlled stirred-tank bioreactors have been performed so far with unicellular microorganisms. We have compared cultivation of industrially relevant oleaginous filamentous fungi and microalga in a Duetz-microtiter plate system to benchtop and pre-pilot bioreactors. Maximal glucose consumption rate, biomass concentration, lipid content of the biomass, biomass, and lipid yield values showed good scalability for Mucor circinelloides (less than 20% differences) and Mortierella alpina (less than 30% differences) filamentous fungi. Maximal glucose consumption and biomass production rates were identical for Crypthecodinium cohnii in microtiter plate and benchtop bioreactor. Most likely due to shear stress sensitivity of this microalga in stirred bioreactor, biomass concentration and lipid content of biomass were significantly higher in the microtiter plate system than in the benchtop bioreactor. Still, fermentation results obtained in the Duetz-microtiter plate system for Crypthecodinium cohnii are encouraging compared to what has been reported in literature. Good reproducibility (coefficient of variation less than 15% for biomass growth, glucose consumption, lipid content, and pH) were achieved in the Duetz-microtiter plate system for Mucor circinelloides and Crypthecodinium cohnii. Mortierella alpina cultivation reproducibility might be improved with inoculation optimization. In conclusion, we have presented suitability of the Duetz-microtiter plate system for the reproducible, scalable, and cost-efficient high-throughput screening of oleaginous microorganisms.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.