ArticleBiotechnology for biofuels2018
Single cell assessment of yeast metabolic engineering for enhanced lipid production using Raman and AFM-IR imaging.
Article in Biotechnology for biofuels, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 43 citations in OpenAlex.
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- Exploring engineering strategies that enhance de novo production of exotic cyclopropane fatty acids in Saccharomyces cerevisiae.Biotechnology journal · 2024Article
- Quantitative Raman chemical imaging of intracellular drug-membrane aggregates and small molecule drug precipitates in cytoplasmic organelles.Advanced drug delivery reviews · 2023Review
- Morphological and lipid metabolism alterations in macrophages exposed to model environmental nanoplastics traced by high-resolution synchrotron techniques.Frontiers in immunology · 2023Article
- Metabolic Engineering Strategies for Improved Lipid Production and Cellular Physiological Responses in YeastJournal of fungi (Basel, Switzerland) · 2022Review
- Non-invasive Raman spectroscopy for time-resolved in-line lipidomics.RSC advances · 2021Article
- Quantum-enhanced nonlinear microscopy.Nature · 2021Article
- Effect of Selenium on the Growth and Lipid Accumulation of Yarrowia lipolytica Yeast.Biological trace element research · 2021Article
- Visualizing wax ester fermentation in singleBiotechnology for biofuels · 2019Article
- Correlated Materials Characterization via Multimodal Chemical and Functional Imaging.ACS nano · 2018Article
- Impact of bioreactor process parameters and yeast biomass on Raman spectra.Biotechnology progressArticle
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundBiodiesel is a valuable renewable fuel made from derivatized fatty acids produced in plants, animals, and oleaginous microbes. Of the latter, yeasts are of special interest due to their wide use in biotechnology, ability to synthesize fatty acids and store large amounts of triacylglycerols while utilizing non-food carbon sources. While yeast efficiently produce lipids, genetic modification and indeed, lipid pathway metabolic engineering, is usually required for cost-effective production. Traditionally, gas chromatography (GC) is used to measure fatty acid production and to track the success of a metabolic engineering strategy in a microbial culture; here we have employed vibrational spectroscopy approaches at population and single cell level of engineered yeast while simultaneously investigating metabolite levels in subcellular structures.
resultsFirstly, a strong correlation (
conclusionsVibrational spectroscopy analysis allowed the simultaneous measurement of strain variability in metabolite production and impact on cellular structures as a result of different gene introductions or knockouts, within a lipid metabolic engineering strategy and these inform the next steps in comprehensive lipid engineering. Additionally, single cell spectroscopic analysis measures heterogeneity in metabolite production across microbial cultures under genetic modification, an emerging issue for efficient biotechnological production.
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