ArticleScientific reports2018
A standardized flow cytometry network study for the assessment of circulating endothelial cell physiological ranges.
Registry-linked trialOpen access · goldAbstract read
In one paragraph
Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to trial NCT04038827 (Search of Circulating Endothelial Cells of Donor Origin After Allogeneic Hematopoietic Stem Cell Transplantation), which is not on this map. Cited by 31 papers.
0numbers the graph read from it
0cells of the map it votes in
31citing papers in PubMed
1.9field-weighted citation impact, top 13% of its field
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Search of Circulating Endothelial Cells of Donor Origin After Allogeneic Hematopoietic Stem Cell Transplantation: Evaluation for Potential Clinically Relevant Implications in the Context of Graft-Versus-Host Disease
Who cites it
31 citing papers in PubMed, 56 citations in OpenAlex.
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- FACS-Proteomics strategy toward extracellular vesicles single-phenotype characterization in biological fluids: exploring the role of leukocyte-derived EVs in multiple sclerosis.Journal of translational medicine · 2025Article
- LGALS3BP antibody-drug conjugate enhances tumor-infiltrating lymphocytes and synergizes with immunotherapy to restrain neuroblastoma growth.Journal of translational medicine · 2025Article
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- The Future of Kawasaki Disease Diagnosis: Liquid Biopsy May Hold the Key.International journal of molecular sciences · 2024Review
- Multicancer screening test based on the detection of circulating non haematological proliferating atypical cells.Molecular cancer · 2024Article
- Distinctive phenogroup to differentiate diagnosis of cardiac myxoma vs cardiovascular disease examining blood-based circulating cell biomarkers.Scientific reports · 2023Article
- Putative circulating adipose tissue-derived stem cells, obesity, and metabolic syndrome features.Journal of endocrinological investigation · 2023Article
- Are circulating endothelial cells the next target for transcriptome-level pathway analysis in ARDS?American journal of physiology. Lung cellular and molecular physiology · 2023Article
- Resveratrol Derivative Exhibits Marked Antiproliferative Actions, Affecting Stemness in Pancreatic Cancer Cells.International journal of molecular sciences · 2023Article
- Rapid Detection ofInternational journal of molecular sciences · 2022Article
- Minimal residual disease assessment of papillary thyroid carcinoma through circulating tumor cell-based cytology.Cancer medicine · 2022Article
- Extracellular Vesicles in Regenerative Processes Associated with Muscle Injury Recovery of Professional Athletes Undergoing Sub Maximal Strength Rehabilitation.International journal of molecular sciences · 2022Article
- Liquid Biopsy-Guided Interventional Oncology: A Proof of Concept with a Special Focus on Radiotherapy and Radiology.Cancers · 2022Review
- Peptides Regulating Proliferative Activity and Inflammatory Pathways in the Monocyte/Macrophage THP-1 Cell Line.International journal of molecular sciences · 2022Article
- Endogenous PCSK9 may influence circulating CD45Scientific reports · 2021Observational
- Biological insight into the extracellular vesicles in women with and without gestational diabetes.Journal of endocrinological investigation · 2021Article
- Development and Validation of a Random Forest Diagnostic Model of Acute Myocardial Infarction Based on Ferroptosis-Related Genes in Circulating Endothelial Cells.Frontiers in cardiovascular medicine · 2021Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
39 authors at 8 institutions in 1 country.
Paola LanutiDepartment of Medicine and Aging Sciences, University "G.d'Annunzio", Chieti-Pescara, Italy.
Pasquale SimeoneDepartment of Medicine and Aging Sciences, University "G.d'Annunzio", Chieti-Pescara, Italy.
Gianluca RottaBD Biosciences Italia, 20090, Milano, Italy.
Camillo AlmiciLaboratory for Stem Cells Manipulation and Cryopreservation, Department of Transfusion Medicine, Spedali Civili of Brescia, Brescia, Italy.ORCID 0000-0002-9438-2569
Giuseppe AvvisatiHematology, Stem Cell Transplantation, Transfusion Medicine and Cellular Therapy, Department of Medicine, Campus Bio-Medico University Hospital, Roma, Italy.
Rosa AzzaroTransfusion Service, Department of Hematology-Oncology and Stem Cell Transplantation Unit, Napoli, Italy.
Giuseppina BolognaDepartment of Medicine and Aging Sciences, University "G.d'Annunzio", Chieti-Pescara, Italy.
Alfredo BudillonExperimental Pharmacology Unit, Department of Research, Istituto Nazionale Tumori- IRCCS G. Pascale, Napoli, Italy.
Melania Di CerboHematology, Stem Cell Transplantation, Transfusion Medicine and Cellular Therapy, Department of Medicine, Campus Bio-Medico University Hospital, Roma, Italy.
Elena Di GennaroExperimental Pharmacology Unit, Department of Research, Istituto Nazionale Tumori- IRCCS G. Pascale, Napoli, Italy.
Maria Luisa Di MartinoUnit of Internal Medicine, Allergy and Clinical Immunology, Department of Medical Sciences "M. Aresu", University of Cagliari Monserrato, Cagliari, Italy.
Annamaria DiodatoTransfusion Service, Department of Hematology-Oncology and Stem Cell Transplantation Unit, Napoli, Italy.
Paolo DorettoClinical Pathology Laboratory, Department of Laboratory Medicine, AAS5, Pordenone Hospital, Pordenone, Italy.
Eva ErcolinoDepartment of Medicine and Aging Sciences, University "G.d'Annunzio", Chieti-Pescara, Italy.
Alessandra FaldaClinical Pathology Laboratory, Department of Laboratory Medicine, AAS5, Pordenone Hospital, Pordenone, Italy.
Chiara GregorjHematology, Stem Cell Transplantation, Transfusion Medicine and Cellular Therapy, Department of Medicine, Campus Bio-Medico University Hospital, Roma, Italy.
Alessandra LeoneExperimental Pharmacology Unit, Department of Research, Istituto Nazionale Tumori- IRCCS G. Pascale, Napoli, Italy.
Francesca LosaUnit of Internal Medicine, Allergy and Clinical Immunology, Department of Medical Sciences "M. Aresu", University of Cagliari Monserrato, Cagliari, Italy.
Natalia MalaraDepartment of Health Science University "Magna Graecia" of Catanzaro, Catanzaro, Italy.
Mirella MariniLaboratory for Stem Cells Manipulation and Cryopreservation, Department of Transfusion Medicine, Spedali Civili of Brescia, Brescia, Italy.
Pasquale MastrorobertoDepartment of Experimental and Clinical Medicine, University "Magna Graecia" of Catanzaro, Catanzaro, Italy.
Vincenzo MollaceDepartment of Health Science University "Magna Graecia" of Catanzaro, Catanzaro, Italy.
Michele MorelliDepartment of Obstetrics and Gynecology, University "Magna Graecia" of Catanzaro, Catanzaro, Italy.
Emma MuggianuUnit of Internal Medicine, Allergy and Clinical Immunology, Department of Medical Sciences "M. Aresu", University of Cagliari Monserrato, Cagliari, Italy.
Giuseppe MusolinoDepartment of Experimental and Clinical Medicine, University "Magna Graecia" of Catanzaro, Catanzaro, Italy.
Arabella NevaLaboratory for Stem Cells Manipulation and Cryopreservation, Department of Transfusion Medicine, Spedali Civili of Brescia, Brescia, Italy.
Laura PierdomenicoDepartment of Medicine and Aging Sciences, University "G.d'Annunzio", Chieti-Pescara, Italy.
Silvia PinnaUnit of Internal Medicine, Allergy and Clinical Immunology, Department of Medical Sciences "M. Aresu", University of Cagliari Monserrato, Cagliari, Italy.
Giovanna PiovaniDepartment Molecular Medicine and Translational, University of Brescia, Brescia, Italy.
Maria Serena RocaExperimental Pharmacology Unit, Department of Research, Istituto Nazionale Tumori- IRCCS G. Pascale, Napoli, Italy.
Domenico RussoUnit of Blood Diseases and Stem Cell Transplantation, University of Brescia, Brescia, Italy.
Lorenza ScottiDepartment of Statistics and Quantitative Methods, University of Milano-Bicocca, Milano, Italy.
Maria Cristina TirindelliHematology, Stem Cell Transplantation, Transfusion Medicine and Cellular Therapy, Department of Medicine, Campus Bio-Medico University Hospital, Roma, Italy.
Valentina TrunzoDepartment of Health Science University "Magna Graecia" of Catanzaro, Catanzaro, Italy.
Roberta VenturellaDepartment of Obstetrics and Gynecology, University "Magna Graecia" of Catanzaro, Catanzaro, Italy.
Carlo VitaglianoExperimental Pharmacology Unit, Department of Research, Istituto Nazionale Tumori- IRCCS G. Pascale, Napoli, Italy.
Fulvio ZulloDepartment of Obstetrics and Gynecology, University "Magna Graecia" of Catanzaro, Catanzaro, Italy.
Marco MarchisioDepartment of Medicine and Aging Sciences, University "G.d'Annunzio", Chieti-Pescara, Italy. m.marchisio@unich.it.
Sebastiano MisciaDepartment of Medicine and Aging Sciences, University "G.d'Annunzio", Chieti-Pescara, Italy.
Magna Graecia University · ITUniversity of Chieti-Pescara · ITIstituto Nazionale Tumori IRCCS "Fondazione G. Pascale" · ITUniversità Campus Bio-Medico · ITUniversity of Cagliari · ITAzienda Socio Sanitaria Territoriale degli Spedali Civili di Brescia · ITUniversity of Brescia · ITUniversity of Milano-Bicocca · IT
Funding
No grant is acknowledged in the PubMed record.
Abstract
Circulating endothelial cells (CEC) represent a restricted peripheral blood (PB) cell subpopulation with high potential diagnostic value in many endothelium-involving diseases. However, whereas the interest in CEC studies has grown, the standardization level of their detection has not. Here, we undertook the task to align CEC phenotypes and counts, by standardizing a novel flow cytometry approach, within a network of six laboratories. CEC were identified as alive/nucleated/CD45negative/CD34bright/CD146positive events and enumerated in 269 healthy PB samples. Standardization was demonstrated by the achievement of low inter-laboratory Coefficients of Variation (CV
Indexed as
Endothelial CellsAdultBiological Variation, PopulationBlood Cell CountCell SeparationEndothelium, VascularFeasibility StudiesFemaleFlow CytometryHealthy VolunteersHematologyHumansLaboratoriesMaleMiddle AgedReference Values
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Read underepoch 390
Registered trials
NCT04038827ClinicalTrials.gov
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.