Evidence map›Paper›PMID 29643234›Full record

ArticleJournal of virology2018

Visualization of Arenavirus RNA Species in Individual Cells by Single-Molecule Fluorescence

Benjamin R King, Aubin Samacoits, Philip L Eisenhauer, Christopher M Ziegler, Emily A Bruce, Daniel Zenklusen, Christophe Zimmer, Florian Mueller, Jason Botten

Abstract read
In one paragraph

Article in Journal of virology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.

0numbers the graph read from it
0cells of the map it votes in
15citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

15 citing papers in PubMed.

  1. Article
  2. Review
  3. Article
  4. Journal of virology · 2023
    Article
  5. Article
  6. Article
  7. Review
  8. Article
  9. Review
  10. Article
  11. Article
  12. Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Benjamin R KingDepartment of Medicine, Division of Immunobiology, University of Vermont, Burlington, Vermont, USA.ORCID https://orcid.org/0000-0003-2919-3961
Aubin SamacoitsUnité Imagerie et Modélisation, Institut Pasteur, Paris, France.
Philip L EisenhauerDepartment of Medicine, Division of Immunobiology, University of Vermont, Burlington, Vermont, USA.
Christopher M ZieglerDepartment of Medicine, Division of Immunobiology, University of Vermont, Burlington, Vermont, USA.
Emily A BruceDepartment of Medicine, Division of Immunobiology, University of Vermont, Burlington, Vermont, USA.
Daniel ZenklusenDepartement de Biochimie et Médecine Moléculaire, Université de Montréal, Montréal, QC, Canada.
Christophe ZimmerUnité Imagerie et Modélisation, Institut Pasteur, Paris, France.
Florian MuellerUnité Imagerie et Modélisation, Institut Pasteur, Paris, France.
Jason BottenDepartment of Medicine, Division of Immunobiology, University of Vermont, Burlington, Vermont, USA jbotten@uvm.edu.

Funding

VERMONT IMMUNOBIOLOIGY/ INFECTIOUS DISEASES CENTERP20RR021905 · NCRR · UNIVERSITY OF VERMONT & ST AGRIC COLLEGE · PI HILL, JACK · 2006 to 2011
$14.5M
Vermont Immunology/Infectious Diseases CenterP30GM118228 · NIGMS · UNIVERSITY OF VERMONT & ST AGRIC COLLEGE · PI BUDD, RALPH C · 2016 to 2021
$6.8M
Multidisciplinary Training in Lung BiologyT32HL076122 · NHLBI · UNIVERSITY OF VERMONT &ST AGRIC COLLEGE · PI IRVIN, CHARLES G, POYNTER, MATTHEW E · 2004 to 2023
$6.1M
Vermont Immunology/Infectious Diseases Training GrantT32AI055402 · NIAID · UNIVERSITY OF VERMONT &ST AGRIC COLLEGE · PI GARY E WARD · 2005 to 2026
$2.7M
Identification of Novel Arenavirus Protein-Host Cellular Protein InteractionsR21AI088059 · NIAID · UNIVERSITY OF VERMONT & ST AGRIC COLLEGE · PI BOTTEN, JASON W. · 2010 to 2011
$416k
CIHR 366682NCRR NIH HHS P20 RR021905NHLBI NIH HHS T32 HL076122NIAID NIH HHS R21 AI088059NIAID NIH HHS T32 AI055402NIGMS NIH HHS P30 GM118228
6 · The paper itself

Abstract

Lymphocytic choriomeningitis mammarenavirus (LCMV) is an enveloped, negative-strand RNA virus that causes serious disease in humans but establishes an asymptomatic, lifelong infection in reservoir rodents. Different models have been proposed to describe how arenaviruses regulate the replication and transcription of their bisegmented, single-stranded RNA genomes, particularly during persistent infection. However, these models were based largely on viral RNA profiling data derived from entire populations of cells. To better understand LCMV replication and transcription at the single-cell level, we established a high-throughput, single-molecule fluorescence

Indexed as

A549 CellsCell LineGenome, ViralHumansIn Situ Hybridization, FluorescenceLymphocytic choriomeningitis virusRNA ProbesRNA, ViralStaining and LabelingVirus ReplicationRNA ProbesRNA, Viralarenaviruscyclicalgene probesgenome replication and transcriptionkineticsLCMVpersistencesmFISH

Identifiers

PMID29643234
PMCPMC5974494

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.