ArticleGenes2018
In Silico Functional Networks Identified in Fish Nucleated Red Blood Cells by Means of Transcriptomic and Proteomic Profiling.
Article in Genes, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
21 citing papers in PubMed, 49 citations in OpenAlex.
- Transcriptomics of early responses to purifiedFrontiers in immunology · 2024Article
- Innate immune response of rainbow trout erythrocytes to spinycterins expressing a downsized viral fragment of viral haemorrhagic septicaemia virus.Aquaculture (Amsterdam, Netherlands) · 2023Article
- Biofloc Technology in Fish Aquaculture: A Review.Antioxidants (Basel, Switzerland) · 2023Review
- GWAS on Imputed Whole-Genome Sequence Variants Reveal Genes Associated with Resistance toGenes · 2022Article
- Immunomodulatory Lectin-like Peptides for Fish Erythrocytes-Targeting as Potential Antiviral Drug Delivery Platforms.International journal of molecular sciences · 2021Article
- Family-effects in the epigenomic response of red blood cells to a challenge test in the European sea bass (Dicentrarchus labrax, L.).BMC genomics · 2021Article
- Blood Will Tell: What Hematological Analyses Can Reveal About Fish Welfare.Frontiers in veterinary science · 2021Review
- Transcriptome Analysis ofInternational journal of molecular sciences · 2020Article
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- Immune Functions of Erythrocytes in Osteichthyes.Frontiers in immunology · 2020Review
- Investigation of the Potential Mechanism Governing the Effect of theEvidence-based complementary and alternative medicine : eCAM · 2020Article
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- Potential Role of Rainbow Trout Erythrocytes as Mediators in the Immune Response Induced by a DNA Vaccine in Fish.Vaccines · 2019Article
- Article
- The Megalocytivirus RBIV Induces Apoptosis and MHC Class I Presentation in Rock BreamFrontiers in immunology · 2019Article
- IFIT5 Participates in the Antiviral Mechanisms of Rainbow Trout Red Blood Cells.Frontiers in immunology · 2019Article
- Fish Red Blood Cells Modulate Immune Genes in Response to Bacterial Inclusion Bodies Made of TNFα and a G-VHSV Fragment.Frontiers in immunology · 2019Article
- An update of circulating rare cell types in healthy adult peripheral blood: findings of immature erythroid precursors.Annals of translational medicine · 2018Article
- Article
- Antiviral Responses and Biological Concequences ofFrontiers in immunology · 2018Review
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Nucleated red blood cells (RBCs) of fish have, in the last decade, been implicated in several immune-related functions, such as antiviral response, phagocytosis or cytokine-mediated signaling. RNA-sequencing (RNA-seq) and label-free shotgun proteomic analyses were carried out for in silico functional pathway profiling of rainbow trout RBCs. For RNA-seq, a de novo assembly was conducted, in order to create a transcriptome database for RBCs. For proteome profiling, we developed a proteomic method that combined: (a) fractionation into cytosolic and membrane fractions, (b) hemoglobin removal of the cytosolic fraction, (c) protein digestion, and (d) a novel step with pH reversed-phase peptide fractionation and final Liquid Chromatography Electrospray Ionization Tandem Mass Spectrometric (LC ESI-MS/MS) analysis of each fraction. Combined transcriptome- and proteome- sequencing data identified, in silico, novel and striking immune functional networks for rainbow trout nucleated RBCs, which are mainly linked to innate and adaptive immunity. Functional pathways related to regulation of hematopoietic cell differentiation, antigen presentation via major histocompatibility complex class II (MHCII), leukocyte differentiation and regulation of leukocyte activation were identified. These preliminary findings further implicate nucleated RBCs in immune function, such as antigen presentation and leukocyte activation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.