ArticleBiophysical journal2018
Active Mechanics Reveal Molecular-Scale Force Kinetics in Living Oocytes.
Article in Biophysical journal, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 92 citations in OpenAlex.
- Direct mechanical communication of cellular to nuclear shapes in oocytes.Biophysical journal · 2026Article
- Active and probe-free intracellular rheology via phase-sensitive thermoviscous flows.PNAS nexus · 2026Article
- Article
- Accessing activity and viscoelastic properties of artificial and living systems from passive measurement.Nature materials · 2024Article
- Activity-dependent glassy cell mechanics II: Nonthermal fluctuations under metabolic activity.Biophysical journal · 2023Article
- Studying fluctuating trajectories of optically confined passive tracers inside cells provides familiar active forces.Biomedical optics express · 2023Article
- Imaging the subcellular viscoelastic properties of mouse oocytes.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Activity-dependent glassy cell mechanics Ⅰ: Mechanical properties measured with active microrheology.Biophysical journal · 2023Article
- Comparison of translational and rotational modes towards passive rheology of the cytoplasm of MCF-7 cells using optical tweezers.Frontiers in physics · 2023Article
- Two-step nuclear centring by competing microtubule- and actin-based mechanisms in 2-cell mouse embryos.EMBO reports · 2022Article
- Cytoplasmic forces functionally reorganize nuclear condensates in oocytes.Nature communications · 2022Article
- Colossal Power Extraction from Active Cyclic Brownian Information Engines.The journal of physical chemistry letters · 2022Article
- Foregut organ progenitors and their niche display distinct viscoelastic properties in vivo during early morphogenesis stages.Communications biology · 2022Article
- Sculpting tissues by phase transitions.Nature communications · 2022Review
- The force loading rate drives cell mechanosensing through both reinforcement and cytoskeletal softening.Nature communications · 2021Article
- Multi-oscillation microrheologyLab on a chip · 2021Article
- Diffusion of kinesin motors on cargo can enhance binding and run lengths during intracellular transport.Molecular biology of the cell · 2021Article
- Article
- Dynamic actin cross-linking governs the cytoplasm's transition to fluid-like behavior.Molecular biology of the cell · 2020Article
- Probing Intracellular Dynamics Using Fluorescent Carbon Dots Produced by Femtosecond LaserACS omega · 2020Article
Corrections and comments
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Authors and funding
9 authors at 5 institutions in 4 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Active diffusion of intracellular components is emerging as an important process in cell biology. This process is mediated by complex assemblies of molecular motors and cytoskeletal filaments that drive force generation in the cytoplasm and facilitate enhanced motion. The kinetics of molecular motors have been precisely characterized in vitro by single molecule approaches, but their in vivo behavior remains elusive. Here, we study the active diffusion of vesicles in mouse oocytes, where this process plays a key role in nuclear positioning during development, and combine an experimental and theoretical framework to extract molecular-scale force kinetics (force, power stroke, and velocity) of the in vivo active process. Assuming a single dominant process, we find that the nonequilibrium activity induces rapid kicks of duration τ ∼ 300 μs resulting in an average force of F ∼ 0.4 pN on vesicles in in vivo oocytes, remarkably similar to the kinetics of in vitro myosin-V. Our results reveal that measuring in vivo active fluctuations allows extraction of the molecular-scale activity in agreement with single-molecule studies and demonstrates a mesoscopic framework to access force kinetics.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.