ArticleInternational journal of molecular sciences2018
Localization Microscopy of Actin Cytoskeleton in Human Platelets.
Article in International journal of molecular sciences, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- Hybrid Multiphoton Lithography Scaffolds for Nanoscale Mechanobiological Assessment in Microscale Bone Models.Small (Weinheim an der Bergstrasse, Germany) · 2026Article
- Antibiotics Impact the Cytotoxicity and Cytopathic Effect ofInternational journal of molecular sciences · 2025Article
- Advancing Platelet Research Through Live-Cell Imaging: Challenges, Techniques, and Insights.Sensors (Basel, Switzerland) · 2025Review
- New buffer systems for photopainting of single biomolecules.RSC applied interfaces · 2024Article
- Low-Fluorescence Starter for Optical 3D Lithography of Sub-40 nm Structures.ACS applied optical materials · 2023Article
- A guide to molecular and functional investigations of platelets to bridge basic and clinical sciences.Nature cardiovascular research · 2022Article
- CRISPR/Cas9 Genome Editing vs. Over-Expression for Fluorescent Extracellular Vesicle-Labeling: A Quantitative Analysis.International journal of molecular sciences · 2021Article
- Real-time 3D single-molecule localization microscopy analysis using lookup tables.Biomedical optics express · 2021Article
- Super-resolution imaging of platelet-activation process and its quantitative analysis.Scientific reports · 2021Article
- Article
- Identification of Parkinson's disease-related pathways and potential risk factors.The Journal of international medical research · 2020Article
- Potential quality improvement of stochastic optical localization nanoscopy images obtained by frame by frame localization algorithms.Scientific reports · 2020Article
- Statistical analysis of 3D localisation microscopy images for quantification of membrane protein distributions in a platelet clot model.PLoS computational biology · 2020Article
- Imaging Platelet Processes and Function-Current and Emerging Approaches for ImagingFrontiers in immunology · 2020Review
- Cell spread area and traction forces determine myosin-II-based cortex thickness regulation.Biochimica et biophysica acta. Molecular cell research · 2019Article
- Influence of Platelet Lysate on 2D and 3D Amniotic Mesenchymal Stem Cell Cultures.Frontiers in bioengineering and biotechnology · 2019Article
Corrections and comments
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Here, we measure the actin cytoskeleton arrangement of different morphological states of human platelets using a new protocol for photo-switching of rhodamine class fluorophores. A new medium composition was established for imaging the cytoskeleton using Alexa Fluor 488 conjugated to phalloidin. Morphological states of platelets bound to a glass substrate are visualized and quantified by two-dimensional localization microscopy at nanoscopic resolution. Marker-less drift correction yields localization of individual Alexa 488 conjugated to phalloidin with a positional accuracy of 12 nm.
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Registered trials
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