Evidence map›Paper›PMID 29637821›Full record

ArticleCell transplantation2018

Orthotopic Transplantation of Achilles Tendon Allograft in Rats: With or without Incorporation of Autologous Mesenchymal Stem Cells.

Michael Aynardi, Talal Zahoor, Reed Mitchell, Jeffrey Loube, Tyler Feltham, Lumanti Manandhar, Sharada Paudel, Lew Schon, Zijun Zhang

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cell transplantation, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
0.8field-weighted citation impact, top 30% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 11 citations in OpenAlex.

  1. Review
  2. Review
  3. Article
  4. Tendon and Ligament Healing and Current Approaches to Tendon and Ligament Regeneration.Journal of orthopaedic research : official publication of the Orthopaedic Research Society · 2020
    Review
  5. Article
  6. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 4 institutions in 1 country.

Michael Aynardi1 Pennsylvania State University, Hershey, PA, USA.
Talal Zahoor2 Texas Tech University, Permian Basin, TX, USA.
Reed Mitchell3 MedStar Union Memorial Hospital, Baltimore, MD, USA.
Jeffrey Loube3 MedStar Union Memorial Hospital, Baltimore, MD, USA.
Tyler Feltham4 The Johns Hopkins University, Baltimore, MD, USA.
Lumanti Manandhar3 MedStar Union Memorial Hospital, Baltimore, MD, USA.
Sharada Paudel3 MedStar Union Memorial Hospital, Baltimore, MD, USA.
Lew Schon3 MedStar Union Memorial Hospital, Baltimore, MD, USA.
Zijun Zhang3 MedStar Union Memorial Hospital, Baltimore, MD, USA.
MedStar Union Memorial Hospital · USJohns Hopkins University · USPenn State Milton S. Hershey Medical Center · USTexas Tech University · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The biology and function of orthotopic transplantation of Achilles tendon allograft are unknown. Particularly, the revitalization of Achilles allograft is a clinical concern. Achilles allografts were harvested from donor rats and stored at -80 °C. Subcutaneous adipose tissue was harvested from the would-be allograft recipient rats for isolation of mesenchymal stem cells (MSCs). MSCs were cultured with growth differentiation factor-5 (GDF-5) and applied onto Achilles allografts on the day of transplantation. After the native Achilles tendon was resected from the left hind limb of the rats, Achilles allograft, with or without autologous MSCs, was implanted and sutured with calf muscles proximally and calcaneus distally. Animal gait was recorded presurgery and postsurgery weekly. The animals were sacrificed at week 4, and the transplanted Achilles allografts were collected for biomechanical testing and histology. The operated limbs had altered gait. By week 4, the paw print intensity, stance time, and duty cycle (percentage of the stance phase in a step cycle) of the reconstructed limbs were mostly recovered to the baselines recorded before surgery. Maximum load of failure was not different between Achilles allografts, with or without MSCs, and the native tendons. The Achilles allograft supplemented with MSCs had higher cellularity than the Achilles allograft without MSCs. Deposition of fine collagen (type III) fibers was active in Achilles allograft, with or without MSCs, but it was more evenly distributed in the allografts that were incubated with MSCs. In conclusion, orthotopically transplanted Achilles allograft healed with host tissues, regained strength, and largely restored Achilles function in 4 wk in rats. It is therefore a viable option for the reconstruction of a large Achilles tendon defect. Supplementation of MSCs improved repopulation of Achilles allograft, but large animal models, with long-term follow up and cell tracking, may be required to fully appreciate the functional benefits of MSCs.

Indexed as

Achilles TendonAllograftsAnimalsCell DifferentiationFemaleGrowth Differentiation Factor 5MaleMesenchymal Stem CellsMesenchymal Stem Cell TransplantationRatsTransplantation, HomologousGDF5 protein, ratGrowth Differentiation Factor 5Achilles tendonallograftmesenchymal stem cellsrattransplantation

Identifiers

PMID29637821
PMCPMC5898692
OpenAlexW2797203838

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.