ArticlePlant biotechnology journal2018
Chimeric 3' flanking regions strongly enhance gene expression in plants.
Article in Plant biotechnology journal, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 55 papers.
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Who cites it
55 citing papers in PubMed.
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- Causes and consequences of experimental variation in Nicotiana benthamiana transient expression.Nature communications · 2026Article
- Synthetic Biology of Plants and Microbes for Agriculture, Environment, and Future Applications.Chemical reviews · 2026Review
- Development of an ultra-efficient prime editing system in tomato.Nature communications · 2026Article
- Computationally Guided Design of a Soluble, Abundant and Functional AnfH Variant for the Expression of Fe-Only Nitrogenase in Plant Mitochondria.Plant biotechnology journal · 2025Article
- Co-transformation using T-DNA genes from Agrobacterium strain 82.139 enhances regeneration of transgenic shoots in Populus.Plant biotechnology journal · 2025Article
- Through the lens of bioenergy crops: advances, bottlenecks, and promises of plant engineering.The Plant journal : for cell and molecular biology · 2025Review
- A novel geminivirus-derived 3' flanking sequence of terminator mediates the gene expression enhancement.Plant biotechnology journal · 2025Article
- Plant engineering: advances, bottlenecks, and promise.The Plant journal : for cell and molecular biology · 2025Article
- Efficient genome editing in dicot plants using calreticulin promoter-driven CRISPR/Cas system.Molecular horticulture · 2025Article
- Production of recombinant IgA1 with defined mucin-type O-glycans inFrontiers in plant science · 2025Article
- Incorporation of regulatory DNA elements within a viral vector improves recombinant protein expression in plants.Scientific reports · 2024Article
- High-Level Production of a Recombinant Protein inInternational journal of molecular sciences · 2024Article
- Optimized dicot prime editing enables heritable desired edits in tomato and Arabidopsis.Nature plants · 2024Article
- Arabidopsis and maize terminator strength is determined by GC content, polyadenylation motifs and cleavage probability.Nature communications · 2024Article
- Deep learning the cis-regulatory code for gene expression in selected model plants.Nature communications · 2024Article
- Multifactorial analysis of terminator performance on heterologous gene expression in Physcomitrella.Plant cell reports · 2024Article
- Agrobacterium-mediated transfer of the Fusarium graminearum Tri6 gene into barley using mature seed-derived shoot tips as explants.Plant cell reports · 2024Article
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2 authors.
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Abstract
Plants represent a promising platform for the highly scalable production of recombinant proteins. Previously, we identified the tobacco extensin terminator lacking its intron as an element that reduced transcript read-through and improved recombinant protein production in a plant-based system. In this study, we systematically compared nonreplicating plant expression vectors containing over 20 commonly used or newly identified terminators from diverse sources. We found that eight gene terminators enhance reporter gene expression significantly more than the commonly used 35S and NOS terminators. The intronless extensin terminator provided a 13.6-fold increase compared with the NOS terminator. Combining terminators in tandem produced large synergistic effects, with many combinations providing a >25-fold increase in expression. Addition of the tobacco Rb7 or TM6 matrix attachment region (MAR) strongly enhanced protein production when added to most terminators, with the Rb7 MAR providing the greatest enhancement. Using deletion analysis, the full activity of the 1193 bp Rb7 MAR was found to require only a 463-bp region at its 3' end. Combined terminators and MAR together provided a >60-fold increase compared with the NOS terminator alone. These combinations were then placed in a replicating geminiviral vector, providing a total of >150-fold enhancement over the original NOS vector, corresponding to an estimated yield of 3-5 g recombinant protein per kg leaf fresh weight or around 50% of the leaf total soluble protein. These results demonstrate the importance of 3' flanking regions in optimizing gene expression and show great potential for 3' flanking regions to improve DNA-based recombinant protein production systems.
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