ArticleAmerican journal of translational research2018
Protective effects of PI3K/Akt signal pathway induced cell autophagy in rat knee joint cartilage injury.
Article in American journal of translational research, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed, 46 citations in OpenAlex.
- Deciphering the function and mechanism of extracorporeal shock wave therapy in knee osteoarthritis (Review).International journal of molecular medicine · 2026Review
- MYDGF as a telomerase activator and therapeutic target for osteoarthritis.Advanced biotechnology · 2026Article
- Schisandra chinensis polysaccharide attenuates diabetic islet cell injury by promoting autophagy via suppression of the PI3K/AKT/mTOR signaling cascade.Journal of molecular histology · 2026Article
- Cartilage repair: unleashing PRP's potential in organoid models.Cytotechnology · 2025Review
- The Role of Exerkines in the Treatment of Knee Osteoarthritis: From Mechanisms to Exercise Strategies.Orthopaedic surgery · 2025Review
- Extracellular matrix: Dystroglycan interactions-Roles for the dystrophin-associated glycoprotein complex in skeletal tissue dynamics.International journal of experimental pathology · 2025Review
- The effect of Miya on skeletal muscle changes by regulating gut microbiota in rats with osteoarthritis through AMPK pathway.BMC musculoskeletal disorders · 2024Article
- Polydatin protects against articular cartilage degeneration by regulating autophagy mediated by the AMPK/mTOR signaling pathway.Histology and histopathology · 2024Article
- Gubi decoction mitigates knee osteoarthritis via promoting chondrocyte autophagy through METTL3-mediated ATG7 mJournal of cellular and molecular medicine · 2024Article
- Metalloproteins as risk factors for osteoarthritis: improving and understanding causal estimates using Mendelian randomization.Clinical rheumatology · 2024Article
- Mechanism of Wenshen Xuanbi Decoction in the treatment of osteoarthritis based on network pharmacology and experimental verification.The Korean journal of physiology & pharmacology : official journal of the Korean Physiological Society and the Korean Society of Pharmacology · 2024Article
- Potential Mechanisms Underlying the Therapeutic Roles ofCurrent computer-aided drug design · 2024Article
- Transcriptomic and Proteomic Analyses Reveal New Insights into Regulatory Mechanisms of Strontium in Bovine Chondrocytes.Animals : an open access journal from MDPI · 2023Article
- Effects of Prunus Tomentosa Thumb Total Flavones on adjuvant arthritis in rats and regulation of autophagy.Technology and health care : official journal of the European Society for Engineering and Medicine · 2023Article
- Review
- Regulation of circADAMTS6-miR-324-5p-PIK3R3 ceRNA pathway may be a novel mechanism of IL-1β-induced osteoarthritic chondrocytes.Journal of bone and mineral metabolism · 2022Article
- Integrative Analyses of Antler Cartilage Transcriptome and Proteome of Gansu Red Deer (Animals : an open access journal from MDPI · 2022Article
- Corresponding Changes of Autophagy-Related Genes and Proteins in Different Stages of Knee Osteoarthritis: An Animal Model Study.Orthopaedic surgery · 2022Article
- Glycyrrhizin regulates rat TMJOA progression by inhibiting the HMGB1-RAGE/TLR4-NF-κB/AKT pathway.Journal of cellular and molecular medicine · 2022Article
- Integrating Network Pharmacology and Experimental Validation to Explore the Key Mechanism of Gubitong Recipe in the Treatment of Osteoarthritis.Computational and mathematical methods in medicine · 2022Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
As the major reason for limb dysfunction, osteoarthritis (OA) is closely correlated with the level of cellular autophagy. PI3K/Akt is a classical signaling pathway which regulates autophagy, but with unclear roles in OA related cartilage injury. Studying PI3K/Akt induced autophagy in rat knee joint cartilage injury and possible functional mechanism is of critical importance for clinical treatment. This study established a rat knee joint cartilage injury model, in which Akt agonist IGF-1 or autophagy inducer Rapamycin was administrated. Western blot was used to detect the expression level of AKT, phosphorylated AKT, Beclin-1 and LC3-II/I. Formation of autophagosome and lysosome was assessed by transmission electron microscopy. HE, safranin O and toluidine blue staining was used to evaluate cartilage injury. TUNEL staining was performed to measure cell apoptosis. Real-time quantitative PCR measured the expression of cartilage injury indexes such as Aggrecan, Collagen II and MMP13. Compared with normal group, iodacetic acid treatment group showed cartilage injury, whereas AKT activation and autophagy induction groups had significant improvement. mRNA analysis showed enhanced degradation of Aggrecan and Collagen II in AKT activation and autophagy groups with decreased MMP13 mRNA level (P<0.05). Western blot results showed that after AKT activation and autophagy induction, protein levels of Beclin-1 and LC3-II/I were remarkably elevated (P<0.05). TUNEL assay showed significant inhibition of cell apoptosis. In conclusion, activation of PI3K/Akt signal pathway can improve iodacetic acid induced rat knee joint cartilage injury through inducing cell autophagy.
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29636866PMC5883117W2905038803What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.