ArticleScientific reports2018
Plasmodium falciparum strains spontaneously switch invasion phenotype in suspension culture.
Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 34 citations in OpenAlex.
- Microfluidic platform for automatic quantification of malaria parasite invasion under physiological flow conditions.Lab on a chip · 2026Article
- Under conditions of high wall shear stress, several PfEBA and PfRH ligands are important for malariamBio · 2025Article
- Stable Plasmodium falciparum merozoite surface protein-1 allelic diversity despite decreasing parasitaemia in children with multiple malaria infections.Malaria journal · 2025Article
- Application of optical tweezer technology reveals that PfEBA and PfRH ligands, not PfMSP1, play a central role in Plasmodium falciparum merozoite-erythrocyte attachment.PLoS pathogens · 2024Article
- Recent increase in low complexity polygenomic infections and sialic acid-independent invasion pathways in Plasmodium falciparum from Western Gambia.Parasites & vectors · 2023Article
- Variations in the Serum Sialic Acid Profiles of Malaria Patients in Zaria, Nigeria: A Cross-Sectional Study.Acta parasitologica · 2022Article
- Short-term cryopreservation and thawing have minimal effects onFrontiers in cellular and infection microbiology · 2022Article
- Phenotypic characterization of GhanaianFrontiers in immunology · 2022Article
- Biophysical Tools and Concepts Enable Understanding of Asexual Blood Stage Malaria.Frontiers in cellular and infection microbiology · 2022Review
- Continuous Cultures ofMediterranean journal of hematology and infectious diseases · 2021Article
- Localization and function of aExperimental biology and medicine (Maywood, N.J.) · 2021Article
- Adaptive immunity selects against malaria infection blocking mutations.PLoS computational biology · 2020Article
- Actomyosin forces and the energetics of red blood cell invasion by the malaria parasite Plasmodium falciparum.PLoS pathogens · 2020Article
- Comparative analysis of asexual and sexual stage Plasmodium falciparum development in different red blood cell types.Malaria journal · 2020Article
- Investigating a Plasmodium falciparum erythrocyte invasion phenotype switch at the whole transcriptome level.Scientific reports · 2020Article
- Article
- Molecular Characterization and Immuno-Reactivity Patterns of a NovelFrontiers in cellular and infection microbiology · 2020Article
- A forward genetic screen reveals a primary role for Plasmodium falciparum Reticulocyte Binding Protein Homologue 2a and 2b in determining alternative erythrocyte invasion pathways.PLoS pathogens · 2018Article
- Parasite-Related Genetic and Epigenetic Aspects and Host Factors InfluencingFrontiers in cellular and infection microbiology · 2018Review
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
Abstract
The extensive redundancy in the use of invasion ligands by Plasmodium falciparum, and its unique ability to switch between invasion pathways have hampered vaccine development. P. falciparum strains Dd2 and W2mef have been shown to change from sialic acid (SA)-dependent to SA-independent phenotypes when selected on neuraminidase-treated erythrocytes. Following an observation of increasing ability of Dd2 to invade neuraminidase-treated cells when cultured for several weeks, we systematically investigated this phenomenon by comparing invasion phenotypes of Dd2, W2mef and 3D7 strains of P. falciparum that were cultured with gentle shaking (Suspended) or under static (Static) conditions. While Static Dd2 and W2mef remained SA-dependent for the entire duration of the investigation, Suspended parasites spontaneously and progressively switched to SA-independent phenotype from week 2 onwards. Furthermore, returning Suspended cultures to Static conditions led to a gradual reversal to SA-dependent phenotype. The switch to SA-independent phenotype was accompanied by upregulation of the key invasion ligand, reticulocyte-binding homologue 4 (RH4), and the increased invasion was inhibited by antibodies to the RH4 receptor, CR1. Our data demonstrates a novel mechanism for inducing the switching of invasion pathways in P. falciparum parasites and may provide clues for understanding the mechanisms involved.
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