ArticleScientific reports2018
Contrasting epigenetic states of heterochromatin in the different types of mouse pluripotent stem cells.
Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed.
- Developmental stage dominates cell-type identity and reveals a chromatin regulatory function for Rad50 in Drosophila.Nucleic acids research · 2026Article
- Dynamic association of H3K36me3 with pericentromeric heterochromatin regulates its replication time.EMBO reports · 2025Article
- BRD8 Guards the Pluripotent State by Sensing and Maintaining Histone Acetylation.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- H3K9 post-translational modifications regulate epiblast/primitive endoderm specification in rabbit blastocysts.Epigenetics & chromatin · 2025Article
- An RNA-dependent and phase-separated active subnuclear compartment safeguards repressive chromatin domains.Molecular cell · 2024Article
- Defining the chromatin signature of pluripotency.Nature cell biology · 2024Article
- Image analysis workflows to reveal the spatial organization of cell nuclei and chromosomes.Nucleus (Austin, Tex.) · 2022Article
- Epigenetics as "conductor" in "orchestra" of pluripotent states.Cell and tissue research · 2022Review
- H3K27me3 at pericentromeric heterochromatin is a defining feature of the early mouse blastocyst.Scientific reports · 2022Article
- OCT4 activates a Suv39h1-repressive antisense lncRNA to couple histone H3 Lysine 9 methylation to pluripotency.Nucleic acids research · 2022Article
- Satellite repeat transcripts modulate heterochromatin condensates and safeguard chromosome stability in mouse embryonic stem cells.Nature communications · 2022Article
- Elevated retrotransposon activity and genomic instability in primed pluripotent stem cells.Genome biology · 2021Article
- The deubiquitinase Usp9x regulates PRC2-mediated chromatin reprogramming during mouse development.Nature communications · 2021Article
- Organoids in domestic animals: with which stem cells?Veterinary research · 2021Review
- Article
- Organization of the Pluripotent Genome.Cold Spring Harbor perspectives in biology · 2021Review
- Transcriptional Activation of Heterochromatin by Recruitment of dCas9 Activators.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Activation of transcription factor circuity in 2i-induced ground state pluripotency is independent of repressive global epigenetic landscapes.Nucleic acids research · 2020Article
- In vitro capture and characterization of embryonic rosette-stage pluripotency between naive and primed states.Nature cell biology · 2020Article
- Untargeted histone profiling during naive conversion uncovers conserved modification markers between mouse and human.Scientific reports · 2019Article
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10 authors.
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Abstract
Mouse embryonic stem cells (ESCs) and epiblast stem cells (EpiSCs) represent naive and primed pluripotency states, respectively, and are maintained in vitro by specific signalling pathways. Furthermore, ESCs cultured in serum-free medium with two kinase inhibitors (2i-ESCs) are thought to be the ground naïve pluripotent state. Here, we present a comparative study of the epigenetic and transcriptional states of pericentromeric heterochromatin satellite sequences found in these pluripotent states. We show that 2i-ESCs are distinguished from other pluripotent cells by a prominent enrichment in H3K27me3 and low levels of DNA methylation at pericentromeric heterochromatin. In contrast, serum-containing ESCs exhibit higher levels of major satellite repeat transcription, which is lower in 2i-ESCs and even more repressed in primed EpiSCs. Removal of either DNA methylation or H3K9me3 at PCH in 2i-ESCs leads to enhanced deposition of H3K27me3 with few changes in satellite transcript levels. In contrast, their removal in EpiSCs does not lead to deposition of H3K27me3 but rather removes transcriptional repression. Altogether, our data show that the epigenetic state of PCH is modified during transition from naive to primed pluripotency states towards a more repressive state, which tightly represses the transcription of satellite repeats.
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