ArticleParasites & vectors2018
Detection and genotyping of Trypanosoma cruzi from açai products commercialized in Rio de Janeiro and Pará, Brazil.
Article in Parasites & vectors, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers, 1 of them a synthesis that pooled it.
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Who cites it
18 citing papers in PubMed, 1 synthesis or guideline pooled it, 39 citations in OpenAlex.
- Distribution, host origin, and transmission cycles of Trypanosoma cruzi genotypes in the State of Rio Grande do Norte, Brazil.Revista da Sociedade Brasileira de Medicina Tropical · 2025Pooled it
- Bibliometric Insights and Recent Advances in the Science, Technology, and Sustainability of Açaí (Foods (Basel, Switzerland) · 2026Review
- In vitro anti-Trypanosomatid activity and chemical profile of crude extracts of Chromolaena hookeriana and Campuloclinium macrocephalum (Asteraceae).Memorias do Instituto Oswaldo Cruz · 2026Article
- Recent Progress in Thiazole, Thiosemicarbazone, and Semicarbazone Derivatives as Antiparasitic Agents Against Trypanosomatids andMolecules (Basel, Switzerland) · 2025Review
- Epidemiological Scenario of American Trypanosomiasis and Its Socioeconomic and Environmental Relations, Pará, Eastern Brazilian Amazon.Tropical medicine and infectious disease · 2025Article
- Home invasion of triatomines (Hemiptera: Reduviidae) in the urban area of Mato Grosso, Brazil.Revista da Sociedade Brasileira de Medicina Tropical · 2025Article
- Eco-epidemiological aspects and risk factors associated with human Chagas disease in rural areas of the state of Piauí, Brazil.BMC infectious diseases · 2024Article
- Oral Chagas disease outbreak by bacaba juice ingestion: A century after Carlos Chagas' discovery, the disease is still hard to manage.PLoS neglected tropical diseases · 2024Article
- The Development of a One-Step RT-qPCR for the Detection and Quantification of Viable Forms ofInternational journal of molecular sciences · 2024Article
- Validation of the NAT Chagas IVD Kit for the Detection and Quantification ofLife (Basel, Switzerland) · 2023Article
- Molecular characterization ofJournal of parasitic diseases : official organ of the Indian Society for Parasitology · 2022Article
- Emerging and reemerging forms of Trypanosoma cruzi transmission.Memorias do Instituto Oswaldo Cruz · 2022Review
- Lipid and fatty acid metabolism in trypanosomatids.Microbial cell (Graz, Austria) · 2021Review
- Biology of Chagas disease vectors: biological cycle and emergence rates of Rhodnius marabaensis Souza et al., 2016 (Hemiptera, Reduviidae, Triatominae) under laboratory conditions.Parasitology research · 2021Article
- Ready-to-use qPCR for detection ofFood and waterborne parasitology · 2021Article
- Validation of a novel multiplex real-time PCR assay for Trypanosoma cruzi detection and quantification in açai pulp.PloS one · 2021Article
- WHF IASC Roadmap on Chagas Disease.Global heart · 2020Review
- Parasite contamination of berries: Risk, occurrence, and approaches for mitigation.Food and waterborne parasitology · 2018Review
Corrections and comments
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Authors and funding
9 authors at 1 institution in 1 country.
Funding
Abstract
backgroundSeveral cases of food-borne acute Chagas disease (ACD) have been reported in the Brazilian Amazon so far. Up to 2004, the occurrence of ACD by oral transmission, associated with food consumption, was rare. Recent cases of ACD in Brazil have been attributed to the consumption of juice from the açai palm containing reservoir animals or insect vectors waste, infected with Trypanosoma cruzi. This study aimed to determine the T. cruzi contamination rate and to genotype the parasite in food samples prepared from açai, which are commercialized in Rio de Janeiro and the Pará States in Brazil.
methodsThe amplificability of DNA extracted from açai samples, and T. cruzi and Triatominae detection were performed by conventional PCR. Molecular characterization was done by multilocus PCR analysis, to determine the parasite discrete type units (DTUs) based on the size of PCR products in agarose gels, using the intergenic region of the spliced leader (SL), 24 Sα rDNA and nuclear fragment A10 as targets.
resultsFrom the 140 samples of açai-based products analyzed, T. cruzi DNA was detected in 14 samples (10%); triatomine DNA was detected in one of these 14 samples. The parasite genotyping demonstrated that food samples containing açai showed a mixture of T. cruzi DTUs with TcIII, TcV and TcI prevailing.
conclusionsIn this study, the molecular detection and identification of T. cruzi from açai-based manufactured food samples, was performed for the first time. Although parasite DNA is a marker of possible contamination during food manufacturing, our findings do not indicate that açai is a source of Chagas disease via oral transmission per se, as live parasites were not investigated. Nevertheless, a molecular approach could be a powerful tool in the epidemiological investigation of outbreaks, supporting previous evidence that açai-based food can be contaminated with T. cruzi. Furthermore, both food quality control and assessment of good manufacturing practices involving açai-based products can be improved, assuring the safety of açai products.
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