ArticleGigaScience2018
Comparative whole genome DNA methylation profiling of cattle sperm and somatic tissues reveals striking hypomethylated patterns in sperm.
Article in GigaScience, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
44 citing papers in PubMed, 71 citations in OpenAlex.
- Tissue-specific epigenetic regulation of fat metabolism in pigs through integrated analysis of DNA methylation and gene expression networks.Journal of animal science and biotechnology · 2026Article
- Comparison between SNP array and imputed data to estimate population structure and ROH hotspots in horse breeds.BMC genomics · 2025Article
- Photoperiodic responsiveness in the DNA methylation and gene expression in the hypothalamus of ovariectomized and estradiol-treated ewes.BMC genomics · 2025Article
- Genetic regulation of sperm DNA methylation in cattle through meQTL mapping.BMC genomics · 2025Article
- Exploring Tissue- and Sex-Specific DNA Methylation in Cattle Using a Pan-Mammalian Infinium Array.International journal of molecular sciences · 2025Article
- Genome-wide scan for signatures of selection in Hanwoo and Angus cattle using whole-genome sequence data.PloS one · 2025Article
- Whole-genome methylation reveals tissue-specific differences in non-CG methylation in bovine.Zoological research · 2024Article
- Identification of DNA methylation markers for age and Bovine Respiratory Disease in dairy cattle: A pilot study based on Reduced Representation Bisulfite Sequencing.Communications biology · 2024Article
- DNA methylation patterns in patients with asthenospermia and oligoasthenospermia.BMC genomics · 2024Article
- DNA methylation haplotype block signatures responding to Staphylococcus aureus subclinical mastitis and association with production and health traits.BMC biology · 2024Article
- Epigenetic changes to gene pathways linked to male fertility in ex situ black-footed ferrets.Evolutionary applications · 2024Article
- Cold exposure impacts DNA methylation patterns in cattle sperm.Frontiers in genetics · 2024Article
- Coordinated alternation of DNA methylation and alternative splicing of PBRM1 affect bovine sperm structure and motility.Epigenetics · 2023Article
- Effects of DNA Methylation on Gene Expression and Phenotypic Traits in Cattle: A Review.International journal of molecular sciences · 2023Review
- Taming transposable elements in livestock and poultry: a review of their roles and applications.Genetics, selection, evolution : GSE · 2023Review
- Harnessing male germline epigenomics for the genetic improvement in cattle.Journal of animal science and biotechnology · 2023Review
- Comparative epigenomics reveals the impact of ruminant-specific regulatory elements on complex traits.BMC biology · 2022Article
- 3D chromatin architecture and transcription regulation in cancer.Journal of hematology & oncology · 2022Review
- Paternal diet induces transgenerational epigenetic inheritance of DNA methylation signatures and phenotypes in sheep model.PNAS nexus · 2022Article
- Predicting male fertility from the sperm methylome: application to 120 bulls with hundreds of artificial insemination records.Clinical epigenetics · 2022Article
Corrections and comments
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Authors and funding
10 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Background: Although sperm DNA methylation has been studied in humans and other species, its status in cattle is largely unknown. Results: Using whole-genome bisulfite sequencing (WGBS), we profiled the DNA methylome of cattle sperm through comparison with three somatic tissues (mammary gland, brain, and blood). Large differences between cattle sperm and somatic cells were observed in the methylation patterns of global CpGs, pericentromeric satellites, partially methylated domains (PMDs), hypomethylated regions (HMRs), and common repeats. As expected, we observed low methylation in the promoter regions and high methylation in the bodies of active genes. We detected selective hypomethylation of megabase domains of centromeric satellite clusters, which may be related to chromosome segregation during meiosis and their rapid transcriptional activation upon fertilization. We found more PMDs in sperm cells than in somatic cells and identified meiosis-related genes such asKIF2B and REPIN1, which are hypomethylated in sperm but hypermethylated in somatic cells. In addition to the common HMRs around gene promoters, which showed substantial differences between sperm and somatic cells, the sperm-specific HMRs also targeted to distinct spermatogenesis-related genes, including BOLL, MAEL, ASZ1, SYCP3, CTCFL, MND1, SPATA22, PLD6, DDX4, RBBP8, FKBP6, and SYCE1. Although common repeats were heavily methylated in both sperm and somatic cells, some young Bov-A2 repeats, which belong to the SINE family, were hypomethylated in sperm and could affect the promoter structures by introducing new regulatory elements. Conclusions: Our study provides a comprehensive resource for bovine sperm epigenomic research and enables new discoveries about DNA methylation and its role in male fertility.
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